Haynes:TechResources: Difference between revisions

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[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Technical Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''Lab Members''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]]
[[Haynes:Research | <font face="trebuchet ms" style="color:#000000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;
</div><br>
</div><br>


=Technical Resources=
<div style="width: 1000px">
 
=BioBrick Collections=
 
* [https://benchling.com/hayneslab Benchling - Haynes Lab Parts & Plasmids]
* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab iGEM Registry - Haynes Lab Parts]
* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldFVkeDFnYjBYMVFHRm5Ta0ZXaVVGZEE Dr. Haynes' parts and vectors]
* [https://docs.google.com/spreadsheet/ccc?key=0As4aQtxfHElzdG8tYllENlE5OEhnRDNCN0ZoY0xvRnc Dr. Haynes' primers]
* [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdDhDR1hFUXlvSFZleGd2RWhrcW03QXc#gid=2 René's primers, oligos, and parts]
* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0Aj7J45qrNRPzdFp5c201WEwxdDlLcGgzQmsxVnBWS3c#gid=0 Brady's Parts]
<br><br>
 
=Chemicals, Etc.=
 
* [https://docs.google.com/spreadsheet/ccc?key=0AoCP2OrURah2dDJtal9Bc1VDbUNvd0xsRDJXNG03VEE&hl=en_US#gid=0 Chemical Inventory Spreadsheet]: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).<br>
* '''DCB Chemical Stockroom''': Located at Physical Sciences Building G-wing (PSG), "Bateman Storage", ground floor - https://maps.asu.edu/?id=120&mrkIid=62979
* '''Compressed Gas''': Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
* '''Dry Ice''': Located in PSG 106 at the DCB Chemical Stockroom
 
<br>
 
=Facilities, Maintenance & Repairs=
 
* ISTB4 Building Manager - '''Frank N. Clark''' (as of 2014)
* Work Orders - submit work orders through this page: http://webtma.asu.edu/ASUServiceRequest/Default.aspx
* Repairs: small instruments - submit work orders to the [http://ets.fulton.asu.edu/machine-shop/instrument-prototype-machine-shop Instrument & Prototype Machine Shop] through this page: https://fultonapps.asu.edu/shop/
 
<br>


==BioBrick Collections==
=Literature=


* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab Haynes Lab Parts]
* [https://login.ezproxy1.lib.asu.edu/login?url=http://www.ncbi.nlm.nih.gov/sites/entrez?otool=azuazslib PubMed via ASU Libraries] - navigating to PubMed through the [http://lib.asu.edu/ ASU Libraries website] will give you ASU password-protected access to scientific journals, free of charge


<br>
<br>


==Chemicals==
=Ordering & Shipping=


* [https://docs.google.com/spreadsheet/ccc?key=0AoCP2OrURah2dDJtal9Bc1VDbUNvd0xsRDJXNG03VEE&hl=en_US#gid=0 Chemical Inventory Spreadsheet]: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).<br>
* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldG1zUm9zX1YxZ3dFMWZ4VV9XZUJJMUE&usp=sharingOrdering Spreadsheet - CURRENT]: Requests for consumables, DNA synthesis, and small instruments are listed here
* '''Ordering Spreadsheets - ARCHIVED''' (For information purposes only. Do not edit these)
** [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHNPcDlwb2VteXBhMnJmVWNXaXVoTmc&usp=sharing 2013-2014]
** [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHkySmt3TTUyLTBMUGlsSk5zdGYwb1E 2011-2013]
* [http://cfo.asu.edu/purchasing-sunrise SunRISE Ordering System]: Authorized lab members can log-in to SunRISE to place orders
* '''DNA Sequencing - SOLS DNA Lab''' {{hide|
** Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
** [http://sols.asu.edu/labs/dna_lab/serv.php Ordering Information]
** [http://sols.asu.edu/labs/dna_lab/forms.php Order Forms]
}}
* '''DNA Sequencing - DNASU @Biodesign'''
** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL
** [http://sequencing.biodesign.asu.edu/ Ordering Information]
** [https://dnatools.biodesign.asu.edu/cgi-bin/dna/password.cgi Log in to Place an Order or Retrieve Results]
* Central Receiving - where large items/ equipment arrives. Call Louie: 480-965-1083


<br>
<br>


==Ordering==
=Office Supplies & Poster Printing=
 
* Poster printing
** '''ASU Vislab''' - [http://vislab.asu.edu/]
 
=Safety=
* [http://openwetware.org/wiki/Image:HaynesLabSOP2014_v2.pdf Standard Operating Procedures (SOP)]  - PDF of the current SOP. Read this immediately after joining the Haynes lab.
* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU]
* [http://ehsaweb.asu.edu/ Biohazard & Chemical Waste Pick-up Request]: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
* [http://ehsaweb.asu.edu/ Individual Training Record]: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
 
<br><br>
 
=Shared Equipment=


* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHkySmt3TTUyLTBMUGlsSk5zdGYwb1E Ordering Spreadsheet]: Order requests for consumables, DNA synthesis, and small instruments are listed here
[[Haynes:Equipment Signup | Sign-up Calendar]]<br>
* [
for all shared instruments


==Shared Equipment==


'''Burrrt Reynolds: -150°C Cryogenic Freezer'''<br>
'''Burrrt Reynolds: -150°C Cryogenic Freezer'''<br>
Update: Burrrt is currently offline. He will be up and running after our move to ISTB4 in Spring 2012.
Model: Sanyo MDFC2156VANC<br>
Location: ISTB4, room 231, linear equipment room<br>
Status: Burrrt is up and running!




'''Lucille: Light Cycler 480'''<br>
'''Lucille: Light Cycler 480'''<br>
Update: The lab will be purchasing a permanent unit this year. Expected arrival is December '11/ January '12. In the mean time, a demo unit will be available for use.
Model: Roche 5015278001<br>
* [http://www.google.com/calendar/embed?src=asu.edu_7s7m81pvth8183tnlnmim9a7io%40group.calendar.google.com&ctz=America/Phoenix Sign-up calendar]: It is '''mandatory''' that you sign up before using the Light Cycler 480. E-mail [[User:Karmella Haynes|Karmella Haynes]] to gain access to the Google calendar.
Location: ISTB4, room 231, south shared workspace<br>
Status: Online<br>
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?&id=htc_060000 User technical information]
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?&id=htc_060000 User technical information]
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?id=htc_070400 Software downloads]
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?id=htc_070400 Software downloads]
* [http://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp?id=UP030000 Design a UPL assay] - As specific as hydrolysis probe assays (e.g., TaqMan), but costs about the same as SYBR Green.
* [http://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp?id=UP030000 Design a UPL assay] - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost  (comparable to SYBR Green).
 
 
'''Plato: BioTek Plate Reader'''<br>
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy<br>
Location: ISTB4, room 231, south shared workspace<br>
Status: Online<br>
 
 
=Wiki Editing=
 
* [http://openwetware.org/wiki/BME100_f2013:WikiHelp Basic Wiki editing tips] - from the ASU BME100 course
* [[Haynes:WikiTemp_NBplasmids | Plasmid Validation Template Page]] - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
* Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php
 
</div>

Revision as of 11:39, 23 March 2015


BioBrick Collections



Chemicals, Etc.

  • Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
  • DCB Chemical Stockroom: Located at Physical Sciences Building G-wing (PSG), "Bateman Storage", ground floor - https://maps.asu.edu/?id=120&mrkIid=62979
  • Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
  • Dry Ice: Located in PSG 106 at the DCB Chemical Stockroom


Facilities, Maintenance & Repairs


Literature


Ordering & Shipping

  • Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
  • Ordering Spreadsheets - ARCHIVED (For information purposes only. Do not edit these)
  • SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
  • DNA Sequencing - SOLS DNA Lab


Office Supplies & Poster Printing

  • Poster printing
    • ASU Vislab - [1]

Safety



Shared Equipment

Sign-up Calendar
for all shared instruments


Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!


Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online


Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online


Wiki Editing

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