Haynes:TechResources: Difference between revisions
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=BioBrick Collections= | =BioBrick Collections= | ||
* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab Haynes Lab Parts] | * [https://benchling.com/hayneslab Benchling - Haynes Lab Parts & Plasmids] | ||
* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab iGEM Registry - Haynes Lab Parts] | |||
* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldFVkeDFnYjBYMVFHRm5Ta0ZXaVVGZEE Dr. Haynes' parts and vectors] | * [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldFVkeDFnYjBYMVFHRm5Ta0ZXaVVGZEE Dr. Haynes' parts and vectors] | ||
* [https://docs.google.com/spreadsheet/ccc?key=0As4aQtxfHElzdG8tYllENlE5OEhnRDNCN0ZoY0xvRnc Dr. Haynes' primers] | * [https://docs.google.com/spreadsheet/ccc?key=0As4aQtxfHElzdG8tYllENlE5OEhnRDNCN0ZoY0xvRnc Dr. Haynes' primers] | ||
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}} | }} | ||
* '''DNA Sequencing - DNASU @Biodesign''' {{hide| | * '''DNA Sequencing - DNASU @Biodesign''' {{hide| | ||
** Sample submission: | ** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL | ||
** [http://sequencing.biodesign.asu.edu/ Ordering Information] | ** [http://sequencing.biodesign.asu.edu/ Ordering Information] | ||
** [http://dnatools.biodesign.asu.edu/cgi-bin/dna/password.cgi Log in to Place an Order or Retrieve Results] - Log-in with PI's e-mail address and ''chipseq12'' | ** [http://dnatools.biodesign.asu.edu/cgi-bin/dna/password.cgi Log in to Place an Order or Retrieve Results] - Log-in with PI's e-mail address and ''chipseq12'' | ||
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=Safety= | =Safety= | ||
* PDF of the current, long version of the [http://openwetware.org/images/c/cf/Haynes_Lab_SOP_2014.pdf Haynes Lab SOP] | |||
* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU] | * [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU] | ||
* [http://ehsaweb.asu.edu/ Biohazard & Chemical Waste Pick-up Request]: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals) | * [http://ehsaweb.asu.edu/ Biohazard & Chemical Waste Pick-up Request]: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals) | ||
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Location: ISTB4, room 231, south shared workspace<br> | Location: ISTB4, room 231, south shared workspace<br> | ||
Status: Online<br> | Status: Online<br> | ||
=Wiki Editing= | |||
* [http://openwetware.org/wiki/BME100_f2013:WikiHelp Basic Wiki editing tips] - from the ASU BME100 course | |||
* [[Haynes:WikiTemp_NBplasmids | Plasmid Validation Template Page]] - OWW notebook copy-paste code for DNA conc., restriction digests, etc. | |||
* Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php | |||
</div> | </div> | ||
Revision as of 12:42, 3 February 2014
BioBrick Collections
- Benchling - Haynes Lab Parts & Plasmids
- iGEM Registry - Haynes Lab Parts
- Dr. Haynes' parts and vectors
- Dr. Haynes' primers
- René's primers, oligos, and parts
- Brady's Parts
Chemicals, Etc.
- Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
- Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
- Dry Ice: Located in PSG 106 at the DCB Stockroom
Literature
- PubMed via ASU Libraries - navigating to PubMed through the ASU Libraries website will give you ASU password-protected access to scientific journals, free of charge
Ordering
- Ordering Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
- Ordering Spreadsheet - 2011-2013: older orders
- SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
- DNA Sequencing - SOLS DNA Lab
- Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
- Ordering Information
- Order Forms
- DNA Sequencing - DNASU @Biodesign
{{{1}}}
Safety
- PDF of the current, long version of the Haynes Lab SOP
- Environmental Health & Safety at ASU
- Biohazard & Chemical Waste Pick-up Request: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
- Individual Training Record: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
Sign-up Calendar
for all shared instruments
Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!
Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online
- User technical information
- Software downloads
- Design a UPL assay - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green).
Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online
Wiki Editing
- Basic Wiki editing tips - from the ASU BME100 course
- Plasmid Validation Template Page - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
- Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php
