Haynes:TechResources: Difference between revisions
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[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br> | [[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br> | ||
[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] | [[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] | ||
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] | |||
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] | |||
[[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] | [[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] | ||
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] | [[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] | ||
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> ''' | [[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] | ||
[[Haynes: | [[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] | ||
[[Haynes: | [[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]] | ||
</div><br> | </div><br> | ||
= | <div style="width: 1000px"> | ||
=BioBrick Collections= | |||
* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab Haynes Lab Parts] | * [https://benchling.com/hayneslab Benchling - Haynes Lab Parts & Plasmids] | ||
* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab iGEM Registry - Haynes Lab Parts] | |||
* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldFVkeDFnYjBYMVFHRm5Ta0ZXaVVGZEE Dr. Haynes' parts and vectors] | |||
* [https://docs.google.com/spreadsheet/ccc?key=0As4aQtxfHElzdG8tYllENlE5OEhnRDNCN0ZoY0xvRnc Dr. Haynes' primers] | |||
* [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdDhDR1hFUXlvSFZleGd2RWhrcW03QXc#gid=2 René's primers, oligos, and parts] | |||
* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0Aj7J45qrNRPzdFp5c201WEwxdDlLcGgzQmsxVnBWS3c#gid=0 Brady's Parts] | |||
<br><br> | |||
=Chemicals, Etc.= | |||
* [https://docs.google.com/spreadsheet/ccc?key=0AoCP2OrURah2dDJtal9Bc1VDbUNvd0xsRDJXNG03VEE&hl=en_US#gid=0 Chemical Inventory Spreadsheet]: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).<br> | |||
* '''Compressed Gas''': Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu) | |||
* '''Dry Ice''': Located in PSG 106 at the DCB Stockroom | |||
<br> | <br> | ||
= | =Literature= | ||
* [https:// | * [https://login.ezproxy1.lib.asu.edu/login?url=http://www.ncbi.nlm.nih.gov/sites/entrez?otool=azuazslib PubMed via ASU Libraries] - navigating to PubMed through the [http://lib.asu.edu/ ASU Libraries website] will give you ASU password-protected access to scientific journals, free of charge | ||
<br> | <br> | ||
=Ordering= | |||
* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx- | * [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHNPcDlwb2VteXBhMnJmVWNXaXVoTmc&usp=sharing Ordering Spreadsheet - CURRENT]: Requests for consumables, DNA synthesis, and small instruments are listed here | ||
* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHkySmt3TTUyLTBMUGlsSk5zdGYwb1E Ordering Spreadsheet - 2011-2013]: older orders | |||
* [http://cfo.asu.edu/purchasing-sunrise SunRISE Ordering System]: Authorized lab members can log-in to SunRISE to place orders | * [http://cfo.asu.edu/purchasing-sunrise SunRISE Ordering System]: Authorized lab members can log-in to SunRISE to place orders | ||
* '''DNA Sequencing | * '''DNA Sequencing - SOLS DNA Lab''' {{hide| | ||
** Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube | |||
** [http://sols.asu.edu/labs/dna_lab/serv.php Ordering Information] | ** [http://sols.asu.edu/labs/dna_lab/serv.php Ordering Information] | ||
** [http://sols.asu.edu/labs/dna_lab/forms.php Order Forms] | ** [http://sols.asu.edu/labs/dna_lab/forms.php Order Forms] | ||
* '''DNA Sequencing | }} | ||
* '''DNA Sequencing - DNASU @Biodesign''' {{hide| | |||
** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL | |||
** [http://sequencing.biodesign.asu.edu/ Ordering Information] | ** [http://sequencing.biodesign.asu.edu/ Ordering Information] | ||
** [http://dnatools.biodesign.asu.edu/ | ** [http://dnatools.biodesign.asu.edu/cgi-bin/dna/password.cgi Log in to Place an Order or Retrieve Results] - Log-in with PI's e-mail address and ''chipseq12'' | ||
}} | |||
<br><br> | |||
=Safety= | |||
* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU] | |||
* [http://ehsaweb.asu.edu/ Biohazard & Chemical Waste Pick-up Request]: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals) | |||
* [http://ehsaweb.asu.edu/ Individual Training Record]: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID. | |||
<br><br> | <br><br> | ||
=Shared Equipment= | |||
[[Haynes:Equipment Signup | Sign-up Calendar]]<br> | |||
for all shared instruments | |||
'''Burrrt Reynolds: -150°C Cryogenic Freezer'''<br> | '''Burrrt Reynolds: -150°C Cryogenic Freezer'''<br> | ||
Model: Sanyo MDFC2156VANC<br> | |||
Location: ISTB4, room 231, linear equipment room<br> | Location: ISTB4, room 231, linear equipment room<br> | ||
Status: Burrrt is | Status: Burrrt is up and running! | ||
'''Lucille: Light Cycler 480'''<br> | '''Lucille: Light Cycler 480'''<br> | ||
Model: Roche 5015278001<br> | |||
Location: ISTB4, room 231, south shared workspace<br> | Location: ISTB4, room 231, south shared workspace<br> | ||
Status: Online<br> | Status: Online<br> | ||
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?&id=htc_060000 User technical information] | * [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?&id=htc_060000 User technical information] | ||
* [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?id=htc_070400 Software downloads] | * [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?id=htc_070400 Software downloads] | ||
* [http://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp?id=UP030000 Design a UPL assay] - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green). | * [http://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp?id=UP030000 Design a UPL assay] - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green). | ||
'''Plato: BioTek Plate Reader'''<br> | |||
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy<br> | |||
Location: ISTB4, room 231, south shared workspace<br> | |||
Status: Online<br> | |||
=Wiki Editing= | |||
* [http://openwetware.org/wiki/BME100_f2013:WikiHelp Basic Wiki editing tips] - from the ASU BME100 course | |||
* [[Haynes:WikiTemp_NBplasmids | Plasmid Validation Template Page]] - OWW notebook copy-paste code for DNA conc., restriction digests, etc. | |||
</div> |
Revision as of 16:05, 12 September 2013
BioBrick Collections
- Benchling - Haynes Lab Parts & Plasmids
- iGEM Registry - Haynes Lab Parts
- Dr. Haynes' parts and vectors
- Dr. Haynes' primers
- René's primers, oligos, and parts
- Brady's Parts
Chemicals, Etc.
- Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
- Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
- Dry Ice: Located in PSG 106 at the DCB Stockroom
Literature
- PubMed via ASU Libraries - navigating to PubMed through the ASU Libraries website will give you ASU password-protected access to scientific journals, free of charge
Ordering
- Ordering Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
- Ordering Spreadsheet - 2011-2013: older orders
- SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
- DNA Sequencing - SOLS DNA Lab
- Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
- Ordering Information
- Order Forms
- DNA Sequencing - DNASU @Biodesign
{{{1}}}
Safety
- Environmental Health & Safety at ASU
- Biohazard & Chemical Waste Pick-up Request: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
- Individual Training Record: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
Sign-up Calendar
for all shared instruments
Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!
Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online
- User technical information
- Software downloads
- Design a UPL assay - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green).
Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online
Wiki Editing
- Basic Wiki editing tips - from the ASU BME100 course
- Plasmid Validation Template Page - OWW notebook copy-paste code for DNA conc., restriction digests, etc.