Haynes:Protocols

   Home     Contact     Protocols     Technical Resources     People     Publications     Research     Internal Info

Protocols

BioBrick Assembly

• BioBrick assembly overview
• Making DNA parts for standardized assembly

Cell Culture: Bacteria

• Chemically competent cell prep

Cell Culture: Mammalian

• Coming soon

Resources at OpenWetWare

• Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.

Recipes

Bromo-Blue/X-cyanol Loading Buffer, 10X [show][hide]

Formula: [30% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol] Volume: 20 mL

• 50% glycerol, 12 mL
• Bromophenol blue, 250 mg
• Xylene cyanol, 250 mg

Bring up to total volume with dH₂O.

LB Broth (Lennox) [show][hide]

Volume: 1 L

• Caesin tryptone, 10 g
• Yeast extract, 5 g
• NaCl, 5 g
• 1 N NaOH, 1 mL

Dissolve in 1000 mL dH₂O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.