Haynes:Protocols
From OpenWetWare
Protocols
BioBrick Assembly
Cell Culture: Bacteria
Cell Culture: Mammalian
- Coming soon
Resources at OpenWetWare
- Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.
Recipes
Bromo-Blue/X-cyanol Loading Buffer, 10X
Formula: [30% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol] Volume: 20 mL
- 50% glycerol, 12 mL
- Bromophenol blue, 250 mg
- Xylene cyanol, 250 mg
Bring up to total volume with dH2O.
LB Broth (Lennox)
Volume: 1 L
- Caesin tryptone, 10 g
- Yeast extract, 5 g
- NaCl, 5 g
- 1 N NaOH, 1 mL
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
