Haynes:Protocols: Difference between revisions
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| Line 33: | Line 33: | ||
|- | |- | ||
| NaCl* || 5 g | | NaCl* || 5 g | ||
|- | |||
| 1 N NaOH || 1 mL | |||
|- | |||
| || 1000 mL | |||
|} | |} | ||
* | * Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. | ||
* Cool to room temperature before adding antibiotics | |||
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl. | |||
Revision as of 20:24, 18 January 2012
Protocols
BioBrick Assembly
Cell Culture: Bacteria
Cell Culture: Mammalian
- Coming soon
Recipes
| LB Broth (Lennox) | |
| Caesin tryptone* | 10 g |
| Yeast extract* | 5 g |
| NaCl* | 5 g |
| 1 N NaOH | 1 mL |
| 1000 mL |
- Dissolve in 1000 mL dH2O. Autoclave to sterilize.
- Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
