Haynes:Protocols

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(Recipes)
(Recipes)
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=Recipes=
=Recipes=
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'''LB Broth (Lennox)''' {{hide|  
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'''Bromo-Blue/X-cyanol Loading Buffer, 10X''' {{hide|  
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Formula: [30% glycerol; 12.5 mg/ml bromophenol blue; 12.5 mg/ml xylene cyanol]
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Volume: 20 mL
* Caesin tryptone, 10 g
* Caesin tryptone, 10 g
* Yeast extract, 5 g
* Yeast extract, 5 g
* NaCl, 5 g
* NaCl, 5 g
* 1 N NaOH, 1 mL
* 1 N NaOH, 1 mL
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Final volume: 1 L<br>Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
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Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
}}
}}
'''LB Broth (Lennox)''' {{hide|  
'''LB Broth (Lennox)''' {{hide|  
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Volume: 1 L
* Caesin tryptone, 10 g
* Caesin tryptone, 10 g
* Yeast extract, 5 g
* Yeast extract, 5 g
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{| class="wikitable" width="300px"
 
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| '''LB Broth (Lennox)''' || &nbsp;
 
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| Caesin tryptone* || 10 g
 
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|-
 
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| Yeast extract* || 5 g
 
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| NaCl* || 5 g
 
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| 1 N NaOH || 1 mL
 
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| &nbsp; || 1000 mL
 
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|}
 
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* Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize.
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</div>
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* Cool to room temperature before adding antibiotics
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* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
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Revision as of 13:45, 19 January 2012

link=Haynes_Lab
link=http://engineering.asu.edu/

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Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Resources at OpenWetWare

  • Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.




Recipes

Bromo-Blue/X-cyanol Loading Buffer, 10X

Formula: [30% glycerol; 12.5 mg/ml bromophenol blue; 12.5 mg/ml xylene cyanol] Volume: 20 mL

  • Caesin tryptone, 10 g
  • Yeast extract, 5 g
  • NaCl, 5 g
  • 1 N NaOH, 1 mL

Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.

LB Broth (Lennox)

Volume: 1 L

  • Caesin tryptone, 10 g
  • Yeast extract, 5 g
  • NaCl, 5 g
  • 1 N NaOH, 1 mL

Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.


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