Haynes:Protocols
From OpenWetWare
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=Recipes= | =Recipes= | ||
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| + | '''LB Broth (Lennox)''' {{hide| | ||
| + | * Caesin tryptone, 10 g | ||
| + | * Yeast extract, 5 g | ||
| + | * NaCl, 5 g | ||
| + | * 1 N NaOH, 1 mL | ||
| + | Final volume: 1 L<br>Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl. | ||
| + | }} | ||
| + | |||
| + | '''LB Broth (Lennox)''' {{hide| | ||
| + | * Caesin tryptone, 10 g | ||
| + | * Yeast extract, 5 g | ||
| + | * NaCl, 5 g | ||
| + | * 1 N NaOH, 1 mL | ||
| + | Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl. | ||
| + | }} | ||
| + | |||
| + | </div> | ||
{| class="wikitable" width="300px" | {| class="wikitable" width="300px" | ||
Revision as of 14:42, 19 January 2012
Protocols
BioBrick Assembly
Cell Culture: Bacteria
Cell Culture: Mammalian
- Coming soon
Resources at OpenWetWare
- Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.
Recipes
LB Broth (Lennox)
- Caesin tryptone, 10 g
- Yeast extract, 5 g
- NaCl, 5 g
- 1 N NaOH, 1 mL
Final volume: 1 L
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
LB Broth (Lennox)
- Caesin tryptone, 10 g
- Yeast extract, 5 g
- NaCl, 5 g
- 1 N NaOH, 1 mL
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
| LB Broth (Lennox) | |
| Caesin tryptone* | 10 g |
| Yeast extract* | 5 g |
| NaCl* | 5 g |
| 1 N NaOH | 1 mL |
| 1000 mL |
- Dissolve in 1000 mL dH2O. Autoclave to sterilize.
- Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
