Haynes:Protocols

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=Recipes=
=Recipes=
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'''LB Broth (Lennox)''' {{hide|
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* Caesin tryptone, 10 g
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* Yeast extract, 5 g
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* NaCl, 5 g
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* 1 N NaOH, 1 mL
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Final volume: 1 L<br>Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
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}}
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'''LB Broth (Lennox)''' {{hide|
 +
* Caesin tryptone, 10 g
 +
* Yeast extract, 5 g
 +
* NaCl, 5 g
 +
* 1 N NaOH, 1 mL
 +
Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
 +
}}
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 +
</div>
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{| class="wikitable" width="300px"

Revision as of 14:42, 19 January 2012

link=Haynes_Lab
link=http://engineering.asu.edu/

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Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Resources at OpenWetWare

  • Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.




Recipes

LB Broth (Lennox)

  • Caesin tryptone, 10 g
  • Yeast extract, 5 g
  • NaCl, 5 g
  • 1 N NaOH, 1 mL

Final volume: 1 L
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.

LB Broth (Lennox)

  • Caesin tryptone, 10 g
  • Yeast extract, 5 g
  • NaCl, 5 g
  • 1 N NaOH, 1 mL

Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.

LB Broth (Lennox)  
Caesin tryptone* 10 g
Yeast extract* 5 g
NaCl* 5 g
1 N NaOH 1 mL
  1000 mL
  • Dissolve in 1000 mL dH2O. Autoclave to sterilize.
  • Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
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