Haynes:Protocols
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'''Cell Culture: Mammalian''' | '''Cell Culture: Mammalian''' | ||
* Coming soon | * Coming soon | ||
| + | |||
| + | '''Resources at OpenWetWare''' | ||
| + | * [http://openwetware.org/wiki/Materials Materials]: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc. | ||
Revision as of 14:21, 19 January 2012
Protocols
BioBrick Assembly
Cell Culture: Bacteria
Cell Culture: Mammalian
- Coming soon
Resources at OpenWetWare
- Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.
Recipes
| LB Broth (Lennox) | |
| Caesin tryptone* | 10 g |
| Yeast extract* | 5 g |
| NaCl* | 5 g |
| 1 N NaOH | 1 mL |
| 1000 mL |
- Dissolve in 1000 mL dH2O. Autoclave to sterilize.
- Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
