Haynes:Protocols

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(Protocols)
(Recipes)
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| NaCl* || 5 g
| NaCl* || 5 g
 +
|-
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| 1 N NaOH || 1 mL
 +
|-
 +
|   || 1000 mL
|}
|}
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* Or 25 g
+
* Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize.
 +
* Cool to room temperature before adding antibiotics
 +
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.

Revision as of 22:24, 18 January 2012

link=Haynes_Lab
link=http://engineering.asu.edu/

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Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Recipes

LB Broth (Lennox)  
Caesin tryptone* 10 g
Yeast extract* 5 g
NaCl* 5 g
1 N NaOH 1 mL
  1000 mL
  • Dissolve in 1000 mL dH2O. Autoclave to sterilize.
  • Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
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