Haynes:MamCultureMedia
From OpenWetWare
| Cell line | Incomplete media | Supplements for complete media (required) | Transgene selection |
| U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep | --- |
| K-562 (suspension) |
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C
