Haynes:MamCultureMedia: Difference between revisions
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| '''Cell Type''' || '''Genotype''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Antibiotic(s) for maintenance''' || '''Antibiotic(s) for selection''' | | '''Cell Type''' || '''Genotype''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Antibiotic(s) for maintenance''' || '''Antibiotic(s) for selection''' | ||
|- | |- | ||
| U2OS (adherent) || 126-1 || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin || ## ug/mL puromycin | | U2OS (adherent) || 126-1(puro), Flp-in T-REx || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin || ## ug/mL puromycin | ||
|} | |} | ||
==Notes== | ==Notes== | ||
Revision as of 16:50, 27 October 2012
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
| Cell line | Incomplete media | Supplements for complete media (required) |
| HEK293 | DMEM high glucose | 10% FBS, 1 % pen/strep |
| K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1 % pen/strep |
| SK-N-SH (adherent) | Eagle's Minimum Essential Medium (EMEM) | 10% FBS, 1 % pen/strep |
| U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep |
Transgenic Cells
| Cell Type | Genotype | Incomplete media | Supplements for complete media (required) | Antibiotic(s) for maintenance | Antibiotic(s) for selection |
| U2OS (adherent) | 126-1(puro), Flp-in T-REx | McCoy's 5A | 10% FBS, 1 % pen/strep | ## ug/mL puromycin | ## ug/mL puromycin |
Notes
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C
