Haynes:MamCultureMedia: Difference between revisions
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| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | | U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | ||
|- | |- | ||
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) | | K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep | ||
|- | |||
| SK-N-SH (adherent) || Eagle's Minimum Essential Medium (EMEM) || 10% FBS, 1 % pen/strep | |||
|} | |} | ||
Revision as of 16:40, 27 October 2012
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
Cell line | Incomplete media | Supplements for complete media (required) |
U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep |
K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1 % pen/strep |
SK-N-SH (adherent) | Eagle's Minimum Essential Medium (EMEM) | 10% FBS, 1 % pen/strep |
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C