Haynes:MamCultureMedia: Difference between revisions
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(New page: {| class="wikitable" |- | '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Transgene selection''' |- | U2OS (adherent) || McCoy's 5A || 10...) |
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=Mammalian Culture Media= | |||
=="Wild type" stock cells (non-transgenic)== | |||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required) | | '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' | ||
|- | |- | ||
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | | U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | ||
|- | |- | ||
| K-562 (suspension) || | | K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep | ||
|} | |} | ||
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* Filter sterilize all media before use | * Filter sterilize all media before use | ||
* Store all media at 4°C | * Store all media at 4°C | ||
==Transgenic Cells== | |||
Revision as of 16:28, 27 October 2012
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
| Cell line | Incomplete media | Supplements for complete media (required) |
| U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep |
| K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1 % pen/strep |
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C
