Haynes:MamCultureMedia

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("Wild type" stock cells (non-transgenic))
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{| class="wikitable" cellspacing=5px
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| '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance<br>antibiotic(s)''' || '''Selection<br>antibiotic(s)'''
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| '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance<br>antibiotic(s)'''
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|-
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| U2OS (adherent) || 126-1(puro), Flp-in T-REx || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin || ## ug/mL puromycin
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| U2OS (adherent) || Flp-in T-REx || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin
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|-
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| HEK 293 (adherent) || Flp-in T-REx || DMEM || 10% FBS, 1 % pen/strep || ## ug/mL puromycin
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Revision as of 20:58, 19 January 2013

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Contents

Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
HEK293 DMEM high glucose 10% FBS, 1% pen/strep
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1% pen/strep
SK-N-SH (adherent) Eagle's Minimum Essential Medium (EMEM) 10% FBS, 1% pen/strep
U2OS (adherent) McCoy's 5A 10% FBS, 1% pen/strep


Transgenic Cells

Cell Type Genotype Medium Supplements
(required)
Maintenance
antibiotic(s)
U2OS (adherent) Flp-in T-REx McCoy's 5A 10% FBS, 1 % pen/strep ## ug/mL puromycin
HEK 293 (adherent) Flp-in T-REx DMEM 10% FBS, 1 % pen/strep ## ug/mL puromycin


Notes

  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C


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