Haynes:MamCultureMedia

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("Wild type" stock cells (non-transgenic))
("Wild type" stock cells (non-transgenic))
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=="Wild type" stock cells (non-transgenic)==
=="Wild type" stock cells (non-transgenic)==
-
{| class="wikitable"
+
{| class="wikitable" cellspacing=5px
|-
|-
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
|-
|-
-
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep  
+
| HEK293 || DMEM high glucose || 10% FBS, 1% pen/strep
|-
|-
-
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep
+
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1% pen/strep
|-
|-
-
| SK-N-SH (adherent) || Eagle's Minimum Essential Medium (EMEM) || 10% FBS, 1 % pen/strep
+
| SK-N-SH (adherent) || Eagle's Minimum Essential Medium (EMEM) || 10% FBS, 1% pen/strep
 +
|-
 +
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1% pen/strep  
|}
|}
 +
<br>
 +
 +
==Transgenic Cells==
 +
{| class="wikitable" cellspacing=5px
 +
|- valign="top"
 +
| '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance<br>antibiotic(s)''' || '''Selection<br>antibiotic(s)'''
 +
|-
 +
| U2OS (adherent) || 126-1(puro), Flp-in T-REx || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin || ## ug/mL puromycin
 +
|}
 +
 +
<br>
 +
 +
==Notes==
* FBS = fetal bovine serum
* FBS = fetal bovine serum
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* Filter sterilize all media before use
* Filter sterilize all media before use
* Store all media at 4°C
* Store all media at 4°C
-
 
-
==Transgenic Cells==
 
</div>
</div>

Revision as of 23:56, 16 November 2012

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Contents

Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
HEK293 DMEM high glucose 10% FBS, 1% pen/strep
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1% pen/strep
SK-N-SH (adherent) Eagle's Minimum Essential Medium (EMEM) 10% FBS, 1% pen/strep
U2OS (adherent) McCoy's 5A 10% FBS, 1% pen/strep


Transgenic Cells

Cell Type Genotype Medium Supplements
(required)
Maintenance
antibiotic(s)
Selection
antibiotic(s)
U2OS (adherent) 126-1(puro), Flp-in T-REx McCoy's 5A 10% FBS, 1 % pen/strep ## ug/mL puromycin ## ug/mL puromycin


Notes

  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C


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