Haynes:MamCultureMedia

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| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
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|-
 
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| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep
 
|-
|-
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep
|-
|-
| SK-N-SH (adherent) || Eagle's Minimum Essential Medium (EMEM) || 10% FBS, 1 % pen/strep
| SK-N-SH (adherent) || Eagle's Minimum Essential Medium (EMEM) || 10% FBS, 1 % pen/strep
 +
|-
 +
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep
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Revision as of 18:40, 27 October 2012

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Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1 % pen/strep
SK-N-SH (adherent) Eagle's Minimum Essential Medium (EMEM) 10% FBS, 1 % pen/strep
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep


  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C


Transgenic Cells

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