Haynes:Luciferase: Difference between revisions
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(New page: ==Cell Lysate Luciferase Assay== Principal: Cells expressing the ''luciferase'' reporter gene are lysed to release the luciferase enzyme into solution. Note that data on individual cells ...) |
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==Cell Lysate Luciferase Assay | <- [[Haynes:Protocols | Back to Protocols]] | ||
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=Cell Lysate Luciferase Assay= | |||
Principal: Cells expressing the ''luciferase'' reporter gene are lysed to release the luciferase enzyme into solution. Note that data on individual cells is lost using this procedure, but it is useful for measuring relative average levels of reporter gene activity in different cell samples. | Principal: Cells expressing the ''luciferase'' reporter gene are lysed to release the luciferase enzyme into solution. Note that data on individual cells is lost using this procedure, but it is useful for measuring relative average levels of reporter gene activity in different cell samples. | ||
Revision as of 15:29, 3 March 2014
Cell Lysate Luciferase Assay
Principal: Cells expressing the luciferase reporter gene are lysed to release the luciferase enzyme into solution. Note that data on individual cells is lost using this procedure, but it is useful for measuring relative average levels of reporter gene activity in different cell samples.
Kits we use in the lab include:
- Steady-Luc Firefly HTS Assay Kit
Below is a general protocol to help you plan/ prepare for a luciferase assay
