Fall 2013, 11/11/13
Use the following text format EXACTLY as it is shown below...
- (year) Title. Author One, Author Two, and Author Three et al. Journal. Volume:pages. Link.
Summary: Very short explanation of why this paper is relevant/ interesting.
- (2011) Engineering a Photoactivated Caspase-7 for Rapid Induction of Apoptosis. Evan Mills, Xi Chen, Elizabeth Pham, Stanley Wong, and Kevin Truong et al. ACS Synthetic Biology, 1.3:75-82. Link.
Summary: A group from University of Toronto developed a protein that causes rapid apotosis (cell death) of targeted cells.
Open edit mode and copy the example list above. Do not erase the <br><br> tags. Do not use keyboard line returns to space out the numbered list, or else each item will start with the number 1.
ACS Synthetic Biology
- (2013) Generic Metric to Quantify Quorum Sensing Activation Dynamics. Anand Pai, Jaydeep Srimani, Yu Tanouchi, et. al. ACS Synthetic Biology. ePub ahead of print. Link.
Summary: Attempt to represent temporal and dose-dependent quorum sensing behavior with a single metric. Only use Lux system, perturb system to represent diversity.
- (2013) Quorum sensing-modulated AND-gate promoters control gene expression in response to a combination of endogenous and exogenous signals. Jasmine Shong, Cynthia H. Collins. ACS Synthetic Biology. ePub ahead of print. Link.
Summary: Device that is activated with IPTG or Tet and AHL. Use Esa system in combination with well-studied Lac or Tet promoter system.
- (2013) A fluorogenic TMP-tag for high signal-to-background intracellular live cell imaging. Jing C, Cornish VW. ACS Synthetic Biology. 8:1704−1712. Link.
Summary: Possible replacement for luciferase.
- (2013) The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology. Georgios Pothoulakis,†,‡ Francesca Ceroni,†,‡ Benjamin Reeve, et. al.. ACS Synthetic Biology. ePub ahead of print. Link.
Summary: Learn more about spinach as a reporter.
Frontiers in Microbiotechnology
Journal of Biological Engineering
- (2013) Hidden hysteresis – population dynamics can obscure gene network dynamics. Phillip Poisson and Kaustubh D Bhalerao Journal of Biological Engineering . 7:16. Link.
Summary: They use a bistable gene network in E-coli to show that the cells concentration will affect the hysteresis and change the steady state level of gene expression. Higher cell concentration show higher hysteresis due to positive feedback loop effects. This research shows that cell concentration should be considered in designing the synthetic gene circuit for long term application.
- (2013) A survey of enabling technologies in synthetic biology. Linda J Kahl and Drew Endy Journal of Biological Engineering . 7:13. Link.
Summary: This is a survey that they did between synthetic biology researchers to find out their opinions and experiences with available technologies such as public and private registries of biological parts, standard methods for physical assembly of DNA constructs, genomic databases that they've used to move forward they researches. This survey try to show the real potential of these technologies and their actual impacts on synthetic biology researches.
- (2013) A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli . Susanna Zucca12, Lorenzo Pasotti and et al. Journal of Biological Engineering . 7:12. Link.
Summary: In this research they designed an integrative vector which has the BioBricks standard restriction sites and be able to integrate the designed part to the target site in E-coli genome with bacteriophage integration mechanism or homologous recombination.
Journal of Cell Biology
- (2013) Whole-genome screening identifies proteins localized to distinct nuclear bodies. Ka-wing Fong, Yujing Li, Wenqi Wang et al. Journal of Cell Biology. 203 (1): 149. Link.
Summary: Fong et al. performed a genome-wide microscopy-based screening for proteins that form nuclear foci and characterized their localizations using markers of known nuclear bodies. In total, they identified 325 proteins localized to distinct nuclear bodies, including Polycomb Repressive Complex(PRC1 & PRC2)
- (2013) The histone demethylase LSD1/KDM1A promotes the DNA damage response. Nima Mosammaparast1, Haeyoung Kim, Benoit Laurent et al. Journal of Cell Biology. 203 (3): 457. Link.
Summary: They report a role for the histone demethylase LSD1/KDM1A in the DNA damage response (DDR). They show that LSD1 is recruited directly to sites of DNA damage.
Molecular Biology of the Cell
Molecular and Cellular Biology
- (2013) Expression of Polycomb Targets Predicts Breast Cancer Prognosis. Alba Jene-Sanz, Renáta Váraljai, Alexandra V. Vilkova, et. al.. Molecular and Cellular Biology. 33:3951-3961. Link.
Mix of analysis of human tissue samples, publicly available ChIP-seq and microarray data, and cell culture experiments to understand how EZH2 expression, a protein subunit of PRC2, can modify cancer cell behavior and predict patient outcomes. They conclude high expression of EZH2 predicts aggressive phenotype, high expression of PRC2 predicts better patient outcome, low expression of PRC2 predicts metastasis..
- (2013) Variable requirements for DNA-binding proteins at polycomb-dependent repressive regions in human HOX clusters. Woo CJ, Kharchenko PV, Daheron L, et. al. Molecular and Cellular Biology. 33:3274-3285. Link.
Found new target sites from HOXB and HOXC that recruit PcG by using a reporter construct. Experiments done in mesenchymal stem cells. Discovered two DNA-binding proteins, JARID2 and YY1, possibly regulate PcG.
- (2010) Epigenetic modifications in pluripotent and differentiated cells. Alexander Meissner. Nature Biotechnology. 28.10: 1079-1088. Link
Review article that summarizes the major methods of epigenetic modification and their use in manipulating cell states.
- (2013) A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA Strack RL, Disney MD, Jaffrey SR. Nature Methods. [Epub ahead of print]. Link.
Summary: The Jaffrey group was the first to develop and test an RNA that folds into a structure that produces green fluorescence ("Spinach"). In this paper, they report a more stable version, Spinach2, with shows a brighter signal. They used the tag to study the dynamics of "toxic RNA" that is associated with FragileX syndrome.
- (2013) Cas9 as a versatile tool for engineering biology Mali P, Esvelt KM, Church GM. Nature Methods. 10:957-63. Link.
Summary: This article is a perspective piece on the Cas9-guide-RNA methodology.
Nature Molecular Systems Biology
- (2011) Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Nazanin Saeidi, Choon Kit Wong, Tat-Ming Lo, et al. Nature Molecular Systems Biology. 7.521:1-11 Link
A group from Nanyang Technological University, Singapore developed engineered E.coli that sense 3OC-12 HSL from P. aeruginosa and express pyocin toxin. Reduced viable P. aeruginosa cells by 99% and inhibited biofilm formation by 90%.
Public Library of Science Biology (PLoS Biology)
- (2013) Chromatin-Specific Regulation of Mammalian rDNA Transcription by Clustered TTF-I Binding Sites. Sarah D. Diermeier, Attila Németh, Michael Rehli,Ingrid Grummt, Gernot Längst. PLoS Genetics 9:9: 1-12. Link
Determined that clustered binding sites increase the binding affinity of transcription factors in chromatin.
Proceedings of the National Academy of Sciences
- (2013) Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells. Patrick Murphy et. al. Proceedings of the National Academy of Sciences. 110: 19: 7772-7777. Link
Miscellaneous Reviews and Media
2013 iGEM World Championship - Projects of Interest
- (2013) COLISWEEPER: The world's first bacterial minesweeper game. ETH Zurich iGEM Team. iGEM World Championship. Link.
Summary: Biological version of the Minesweeper game. Bacterial spot-cultures (spaced on a grid on agar) represented either mines or number clues (via expression of some combination of colorimetric enzymes). Quorum sensing (Lux) was used to enable the number clue spots to create a certain color depending upon their positioning near one, two, or more mines. Their data included a nice demonstration of AHL-concentration-dependent gene induction.
- (2013) E. teamwork: Engineering a synthetic microbial consortium. Braunschweig iGEM Team. iGEM World Championship. Link.
Summary: Quroum sensing (Las and Rhl) was used to cross-induce ampicillin resistance in cells so that the survival of each relied on eachother. Cell types were "tagged" with pigment expression (blue and pink) in order to quantify the proportion of each in co-culture. The team reported a third strain (labeled yellow) but I only saw data for a two-strain blue/ pink co-culture on their poster.
- (2013) Exosome mediated mammalian cell-cell communication. MIT iGEM Team. iGEM World Championship. Link.
Summary: The Acyl-TyA peptide tag was used to incorporate proteins into mammalian exosomes, which are vesicles that carry "cargo" from one mammalian cell to another. There is data that suggests that cell membrane localization worked, but no data for actual protein delivery (just tests to see if the tag disrupted protein function).
- (2013) The uniCAS toolkit for gene regulation. Freiburg iGEM Team. iGEM World Championship. Link.
Summary: The team used the Cas9 protein/ guide-RNA system to target various transcriptional regulators to genes in mammalian cells. One very cool, straight-forward application was a split transcription activator: Cas9+CRY2 binds DNA, and CIB1+VP16 is required to activate transcription. Blue light stimulates CRY2-CIB1 interaction; as a result, VP16 recruitment to a gene is controlled by light. Note: the Cas9+Vp16 has been published recently.