Haynes:Internal: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(66 intermediate revisions by 2 users not shown)
Line 2: Line 2:
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Research | <font face="trebuchet ms" style="color:#000000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
Line 8: Line 7:
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel Info''' </font>]]
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]]
</div><br>
</div><br>


<div style="width: 1000px">
<div style="width: 1000px">


=Calendar=
<html><iframe src="http://www.google.com/calendar/embed?src=asu.edu_h8h929vntop8tdg1cpvh1aplkc%40group.calendar.google.com&ctz=America/Phoenix" style="border: 0" width="800" height="600" frameborder="0" scrolling="no"></iframe></html>
* Haynes Lab Google Calendar <html><a href="http://www.google.com/calendar/embed?src=asu.edu_h8h929vntop8tdg1cpvh1aplkc%40group.calendar.google.com&ctz=America/Phoenix">link</html>
* Group Lab Meetings: roughly every other Monday starting August 26th; 1:00 pm - 2:00 pm, as of August 2013
* Personal Meetings: members who are working on research projects are expected to meet with Dr. Haynes once every two weeks, as of August 2013
* Birthdays: Add yours to the calendar so we can celebrate!


=Lab Duties=
=Lab Duties=
Do these tasks yourself or recruit others to help you. Your helpers must have all lab [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training] completed.
Officer duties are assigned to postdocs, grad students, and advanced-level undergrads.




'''SPRING 2013'''
'''FALL 2014'''
{| {{table}} width="700"
{| {{table}} width="700"
|- valign="top"
|- valign="top"
| width="150" | '''Hazardous Waste'''  
| width="150" | '''Hazardous Waste Officer'''  
| width="100" | ''not yet assigned''
| width="100" | ---
| autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
| Train volunteers as needed: autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
|- valign="top"
|- valign="top"
| '''Tissue Culture Media Prep'''
| '''Tissue Culture Media Prep'''
| ''not yet assigned''
| Dr. Haynes
| Tissue culture FBS and pen/strep
| Tissue culture FBS and pen/strep, CO<sub>2</sub> incubator water pans
|- valign="top"
|- valign="top"
| '''Shipping & Receiving'''
| '''Shipping & Receiving Officer'''
| James Alling
| Rene Davis
| pick up orders from ECG 334A, enter items received in the ordering spreadsheet, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks)
| Train shipping/ receiving volunteers as needed, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks), help update Trello and ordering spreadsheet
|- valign="top"
|- valign="top"
| '''Safety Officer'''
| '''Safety Officer'''
Line 46: Line 37:
| SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
| SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
|- valign="top"
|- valign="top"
| '''Shared Chemicals'''
| '''Shared Chemicals Officer'''
| Behzad Damadzadeh
| ---
| media prep bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
| media prep bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
|- valign="top"
|- valign="top"
| '''VOLUNTEER DUTIES'''
| '''[[Haynes:Volunteer_Duties | VOLUNTEER DUTIES]]'''
| Undergraduate volunteers
| [https://docs.google.com/a/asu.edu/spreadsheets/d/1_EiqNW0FIlPTwk6bMgQ66_d_buje5ohBaHFxj3cZ6VM/edit?usp=sharing Volunteer Schedule]
| prep LB agar 100 μg/mL ampicillin plates; wash and autoclave glassware, plasticware, & glass beads; prep and autoclave LB liquid media and 50xTAE; prep 70% ethanol and 1x TAE; re-stock autoclaved materials on shelves
| Duties include Glassware & plasticware; Glass beads; [[Haynes:LB_liquid_media | prep and autoclave LB liquid media]] and 50xTAE; 70% ethanol carboy; 1x TAE carboy
|}
|}
<br>
<br>


=Undergraduate Volunteers=
=Undergraduate Volunteers=
Undergraduate volunteers are invited to learn and carry out media and glassware preparation. Volunteers who demonstrate reliability, good technique, and good lab citizenship will be offered opportunities for research projects, either for course credit or FURI.
Undergraduate volunteers are invited to work with researchers as apprentices or support basic lab operations. You must dedicate at least one day and two hours per week, every week, for a whole semester. Volunteers who demonstrate reliability, good technique, and good lab citizenship will be offered opportunities for research projects, either for course credit or a fellowship (e.g., FURI, SOLUR, SynBERC, etc.).<br><br>


'''SPRING CYCLE'''
Review the full '''[[Haynes:Volunteer_Policy | Haynes Lab Volunteer Policy]]''' page at http://openwetware.org/wiki/Haynes:Volunteer_Policy.
* January 1 - February 28: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
* up through mid-March (Spring break): complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]
* Mid-march (after spring break): attend Haynes lab media prep training
* April 30 - spring volunteer cycle ends; continuation through May, June, etc. is welcome.


'''SUMMER CYCLE'''
{| {{table}} width=800px
* May 1 - June 30: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
|- valign="top"
* July 1 - July 15: complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]
| &nbsp; || '''SPRING CYCLE''' || '''SUMMER CYCLE''' || '''FALL CYCLE'''
* Mid-July: attend Haynes lab media prep training
|- valign="top"
* August 30 - summer volunteer cycle ends; continuation through September, October, etc. is welcome.
| width=300 | Requests to join the lab are accepted. Please send requests to karmella dot haynes at asu. You will be asked to complete a Volunteer Interest Survey. || December 1 - January 17 || May 1 - May 12 || July 1  - August 31
 
|- valign="top"
'''FALL CYCLE'''
| width=300 | If your responses are approved by the lab group, you will be invited to engage in an informal interview with ~3 current lab members. || December 1 - January 17  || May 1 - May 12 || July 1 - August 31
* September 1 - October 31: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
|- valign="top"
* November 1 - November 15: complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]
| width=300px | '''Complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]''' by this date. Notify Dr. Haynes via e-mail when all training is completed. || February 7 || June 2 || September 28
* Mid-November: attend Haynes lab media prep training
|- valign="top"
* December 30 - fall volunteer cycle ends; continuation through January, February, etc. is welcome.
| width=300px | [http://openwetware.org/wiki/Haynes:Internal#ISAAC Request ISAAC access] to ISTB4. Safety training is required before your ISAAC request is approved. || February 8 || June 7 || September 28
|- valign="top"
| width=300px | Volunteer cycle ends; continuation through the next cycle is welcome. || May 2 || August 30 || December 15
|}




Line 84: Line 73:


'''INITIAL COURSES'''<br>
'''INITIAL COURSES'''<br>
All '''new''' lab members must take the following courses:<br>
* All lab members and volunteers must take the following courses
* Everyone should complete Compressed Gas to learn about general hazards, but you will only need to complete the Mentor Verification Checklist if you are invited to help with gas cylinders (rare case)


{| {{table}}
{| {{table}}
|-
|-
| Class || Course # || In class or Online
| Topic || Initial Training || In class or Online || Annual Refresher || In class or Online
|-  
|-  
| 1. Laboratory Safety || LABSAFE || In class
| 1. Laboratory Safety || LABSAFE || In class || Laboratory Safety Refresher || Online
|-
|-
| 2. Fire Safety || FIRE || Online
| 2. Fire Safety || Fire Safety - Initial || Online || Fire Safety Refresher || Online
|-
|-
| 3. Compressed Gas - Under Pressure ||   || Online
| 3. Gas Cylinders || Compressed Gas - Under Pressure || Online || N/A || N/A
|-
|-
| 4. Biosafety & Bloodborne Pathogens Initial Training || || Online
| 4. Biosafety & Bloodborne Pathogens || Biosafety & Bloodborne Pathogens Initial Training || Online || Biosafety & Bloodborne Pathogens Refresher Training || Online
|-
|-
| 5. Hazardous Waste Management ||   || Online
| 5. Hazardous Waste || Hazardous Waste Management || Online || Hazardous Waste Management Refresher || Online
|-
|-
| 6. Recombinant DNA Training NIH || || Online
| 6. Synthetic DNA || Recombinant DNA Training (NIH) || Online || N/A || N/A
|-
| 7. SBHSE Lab Safety || Contact Alana LaBelle || In class || N/A || N/A
|}
|}


'''In class training''': Register at the [http://cfo.asu.edu/ehs-training EHS Training Website]. Click "Search Course Catalog/Register for Training." Click "Training Registration" on the EH&S Assistant page and follow the instructions. When you attend class, do not forget to sign the instructor's attendance sheet, or your training will not be kept on record.<br>
'''In class training''': Register at the [http://cfo.asu.edu/ehs-training EHS Training Website]. Click '''Search Course Catalog/Register for Training'''. Click "Training Registration" on the EH&S Assistant page, enter your ASU ID, and select your name. Click "Select from all Available Courses," select the appropriate course at a date and time that work for you, and click "Click to Register for this Class." You should see a yellow page saying you are registered. Click "OK" and confirm that the desired course is listed there. You will also receive a confirmation email. When you attend class, do not forget to sign the instructor's attendance sheet, or your training will not be kept on record.<br>
''Registration errors'': if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.<br>
''Registration errors'': if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.<br>


Line 109: Line 101:
course listed in your "Courses" module. These courses typically include some reading material (a powerpoint online) and a quiz. Read the material and take the 10-question quiz. These should not take longer than about 30 minutes.<br>
course listed in your "Courses" module. These courses typically include some reading material (a powerpoint online) and a quiz. Read the material and take the 10-question quiz. These should not take longer than about 30 minutes.<br>


 
'''Refresher Courses'''<br>
'''REFRESHER COURSES'''<br>
All lab members are required to take annual refresher courses. Reminders will be sent by EHS. Check [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdFV6Qi1CbnEweFhjOHFRa1hMR3NBTWc#gid=0 here] to check your training status. See above for registration instructions under Online training. Email rene dot davis at asu dot edu if the google doc is not accurate or after you complete a training session.
All lab members are required to take annual refresher courses. Reminders will be sent by EHS. Check [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdFV6Qi1CbnEweFhjOHFRa1hMR3NBTWc#gid=0 here] to check your training status. See above for registration instructions under Online training. Email rene dot davis at asu dot edu if the google doc is not accurate or after you complete a training session.
{| {{table}}
|-
| Class || Course # || In class or Online
|-
| 1. Laboratory Safety Annual Refresher Training || n/a || Online
|-
| 2. Fire Safety & Prevention Annual Refresher Training || n/a || Online
|-
| 3. Refresher Biosafety & Bloodborne Pathogens || n/a || Online
|-
| 4. Hazardous Waste Management Refresher || n/a || Online
|}


=ISAAC=
=ISAAC=

Revision as of 12:47, 14 November 2014



Lab Duties

Officer duties are assigned to postdocs, grad students, and advanced-level undergrads.


FALL 2014

Hazardous Waste Officer --- Train volunteers as needed: autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
Tissue Culture Media Prep Dr. Haynes Tissue culture FBS and pen/strep, CO2 incubator water pans
Shipping & Receiving Officer Rene Davis Train shipping/ receiving volunteers as needed, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks), help update Trello and ordering spreadsheet
Safety Officer Rene Davis SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
Shared Chemicals Officer --- media prep bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
VOLUNTEER DUTIES Volunteer Schedule Duties include Glassware & plasticware; Glass beads; prep and autoclave LB liquid media and 50xTAE; 70% ethanol carboy; 1x TAE carboy


Undergraduate Volunteers

Undergraduate volunteers are invited to work with researchers as apprentices or support basic lab operations. You must dedicate at least one day and two hours per week, every week, for a whole semester. Volunteers who demonstrate reliability, good technique, and good lab citizenship will be offered opportunities for research projects, either for course credit or a fellowship (e.g., FURI, SOLUR, SynBERC, etc.).

Review the full Haynes Lab Volunteer Policy page at http://openwetware.org/wiki/Haynes:Volunteer_Policy.

  SPRING CYCLE SUMMER CYCLE FALL CYCLE
Requests to join the lab are accepted. Please send requests to karmella dot haynes at asu. You will be asked to complete a Volunteer Interest Survey. December 1 - January 17 May 1 - May 12 July 1 - August 31
If your responses are approved by the lab group, you will be invited to engage in an informal interview with ~3 current lab members. December 1 - January 17 May 1 - May 12 July 1 - August 31
Complete ALL required Safety Training by this date. Notify Dr. Haynes via e-mail when all training is completed. February 7 June 2 September 28
Request ISAAC access to ISTB4. Safety training is required before your ISAAC request is approved. February 8 June 7 September 28
Volunteer cycle ends; continuation through the next cycle is welcome. May 2 August 30 December 15



Safety Training

INITIAL COURSES

  • All lab members and volunteers must take the following courses
  • Everyone should complete Compressed Gas to learn about general hazards, but you will only need to complete the Mentor Verification Checklist if you are invited to help with gas cylinders (rare case)
Topic Initial Training In class or Online Annual Refresher In class or Online
1. Laboratory Safety LABSAFE In class Laboratory Safety Refresher Online
2. Fire Safety Fire Safety - Initial Online Fire Safety Refresher Online
3. Gas Cylinders Compressed Gas - Under Pressure Online N/A N/A
4. Biosafety & Bloodborne Pathogens Biosafety & Bloodborne Pathogens Initial Training Online Biosafety & Bloodborne Pathogens Refresher Training Online
5. Hazardous Waste Hazardous Waste Management Online Hazardous Waste Management Refresher Online
6. Synthetic DNA Recombinant DNA Training (NIH) Online N/A N/A
7. SBHSE Lab Safety Contact Alana LaBelle In class N/A N/A

In class training: Register at the EHS Training Website. Click Search Course Catalog/Register for Training. Click "Training Registration" on the EH&S Assistant page, enter your ASU ID, and select your name. Click "Select from all Available Courses," select the appropriate course at a date and time that work for you, and click "Click to Register for this Class." You should see a yellow page saying you are registered. Click "OK" and confirm that the desired course is listed there. You will also receive a confirmation email. When you attend class, do not forget to sign the instructor's attendance sheet, or your training will not be kept on record.
Registration errors: if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.

Online training: To register for online training, go here. Scroll to the bottom and click "Enroll" next to the course you need to take. Make sure to enroll in the refresher course if you've already taken the class. After you enroll, go to blackboard and find the course listed in your "Courses" module. These courses typically include some reading material (a powerpoint online) and a quiz. Read the material and take the 10-question quiz. These should not take longer than about 30 minutes.

Refresher Courses
All lab members are required to take annual refresher courses. Reminders will be sent by EHS. Check here to check your training status. See above for registration instructions under Online training. Email rene dot davis at asu dot edu if the google doc is not accurate or after you complete a training session.

ISAAC

Request door access online using the secure system. You must have an ASURITE ID and password.

  • Log in at http://isaacrequest.asu.edu/control.php
  • Click the Request link in the left menu
  • Select ISTB4 (Interdisciplinary Science and Technology Building 4)
  • Select the rooms shown in the table below. Access to these doors will allow you to participate in Haynes Lab research & meetings.
  • Read the guidelines and check the "I have read, understood and will comply with these guidelines." box.
  • Click the [Add] button.
  Bulding Room Sub-Location
ISTB4 1st Floor Perimeter
ISTB4 Conference Rooms (BHSE only)
ISTB4 Autoclaves
ISTB4 1st Floor Dock and LER
ISTB4 2nd Floor LER Lab
ISTB4 231 2nd Floor Wet Lab
ISTB4 231 231 Lab/LER/Elev
ISTB4 431 A2 4th Floor Autoclave (BHSE only)
ISTB4 ISTB4 Entrance Group BHSE