Haynes:Internal: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(43 intermediate revisions by 2 users not shown)
Line 2: Line 2:
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Research | <font face="trebuchet ms" style="color:#000000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
Line 8: Line 7:
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel Info''' </font>]]
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]]
</div><br>
</div><br>


=Calendar=
<div style="width: 1000px">
 
 
=Lab Duties=
Do these tasks yourself or recruit others to help you. Your helpers must have all lab [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training] completed.


[http://www.google.com/calendar/embed?src=asu.edu_h8h929vntop8tdg1cpvh1aplkc%40group.calendar.google.com&ctz=America/Phoenix Google Calendar - Haynes Lab Meetings & Events]
* General Lab Meetings: every other Tuesday starting January 8th; 2:00 pm - 3:00 pm, as of January 2013
* Personal Meetings: members are expected to meet with Dr. Haynes once every two weeks, as of August 2012
* Birthdays: Add yours to the calendar so we can celebrate!
<br>


=Lab Duties (Spring 2013)=
'''SPRING 2013'''
Do these tasks yourself or recruit others to help you. Your helpers must have all lab safety training completed.
{| {{table}} width="700"
{| width="700"
|- valign="top"
|- valign="top"
| width="150" | '''Hazardous Waste'''  
| width="150" | '''Hazardous Waste'''  
| width="100" | Amanda Ispas
| width="100" | Behzad Damadzadeh
| autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
| autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
|- valign="top"
|- valign="top"
| '''Media Prep'''
| '''Tissue Culture Media Prep'''
| Caroline Hom
| Dr. Haynes
| LB Amp agar plates, LB plain agar plates, LB plain liquid, Tissue culture FBS and pen/strep, 50x TAE, TAE and 70% etOH carboys
| Tissue culture FBS and pen/strep, CO<sub>2</sub> incubator water pans
|- valign="top"
|- valign="top"
| '''Shipping & Receiving'''
| '''Shipping & Receiving'''
| James Alling
| Rene Davis
| pick up orders from ECG 334A, enter items received in the ordering spreadsheet, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks)
| pick up orders from ECG 334A, enter items received in the ordering spreadsheet, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks)
|- valign="top"
|- valign="top"
Line 38: Line 36:
| Rene Davis
| Rene Davis
| SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
| SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
|- valign="top"
| '''Glassware'''
| Brady Laughlin
| wash and autoclave glassware, glass beads, re-stock glassware on shelves
|- valign="top"
|- valign="top"
| '''Shared Chemicals'''
| '''Shared Chemicals'''
| Behzad Damadzadeh
| Behzad Damadzadeh
| chemical bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
| media prep bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
|- valign="top"
| '''VOLUNTEER DUTIES'''
| Undergraduate volunteers
| [[Haynes:LBagarAmp | LB agar 100 μg/mL ampicillin plates]]; [[Haynes:LBagarAmp | LB agar plain plates]]; Glassware & plasticware; Glass beads; prep and autoclave LB liquid media and 50xTAE; 70% ethanol carboy; 1x TAE carboy
|}
|}
<br>
<br>


'''Undergraduate Volunteers'''<br>
=Undergraduate Volunteers=
Contact the following undergraduate volunteers (via the haynes-lab-volunteers google group) during the following times to help you with lab duties (as needed).<br>
Undergraduate volunteers are invited to learn and carry out media and glassware preparation. Hours for volunteering are on an on-call basis. You will receive a request to work in the lab via a volunteers' mailing list. Volunteers who demonstrate reliability, good technique, and good lab citizenship will be offered opportunities for research projects, either for course credit or a fellowship (e.g., FURI, SOLUR, SynBERC, etc.).
 
Mondays
 
 
Tuesdays
* 9:00 am - 10:00 am, Jan Simper
* 12:00 pm - 1:00 pm, Jan Simper
 


Wednesdays
'''SPRING CYCLE'''
* December 1 - January 17: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
* March 1: '''Complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]''' by this date. Notify Dr. Haynes via e-mail when all training is completed.
* March 1: [http://openwetware.org/wiki/Haynes:Internal#ISAAC Request ISAAC access] to ISTB4. Safety training is required before your ISAAC request is approved.
* March 1: Attend Haynes lab media prep training.
* May 2 - spring volunteer cycle ends; continuation through May, June, etc. is welcome.


'''SUMMER CYCLE'''
* May 1 - June 30: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
* July 1 - July 15: complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]
* Mid-July: attend Haynes lab media prep training
* August 30 - summer volunteer cycle ends; continuation through September, October, etc. is welcome.


Thursdays
'''FALL CYCLE'''
* 9:00 am - 12:00 pm, David Barclay
* July 1  - August 31: E-mail Dr. Haynes (karmella dot haynes at asu) to express your interest in volunteering for the lab.
* September 28: '''Complete ALL required [http://openwetware.org/wiki/Haynes:Internal#Safety_Training Safety Training]''' by this date. Notify Dr. Haynes via e-mail when all training is completed.
* September 28: [http://openwetware.org/wiki/Haynes:Internal#ISSAC Request ISAAC access] to ISTB4. Safety training is required before your ISAAC request is approved.
* September 28: Attend Haynes lab media prep training.
* December 15: Fall volunteer cycle ends; continuation through December, onward is welcome.


Fridays
* 2:30 pm - 4:00 pm, Jan Simper


<br>
<br>
Line 75: Line 76:


'''INITIAL COURSES'''<br>
'''INITIAL COURSES'''<br>
All '''new''' lab members must take the following courses:<br>
All '''new''' lab members and volunteers must take the following courses:<br>


{| {{table}}
{| {{table}}
Line 83: Line 84:
| 1. Laboratory Safety || LABSAFE || In class
| 1. Laboratory Safety || LABSAFE || In class
|-
|-
| 2. Fire Safety || FIRE || In class
| 2. Fire Safety || FIRE || Online
|-
|-
| 3. Compressed Gas - Under Pressure ||  || Online
| 3. Compressed Gas - Under Pressure ||  || Online
Line 97: Line 98:
''Registration errors'': if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.<br>
''Registration errors'': if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.<br>


'''Online training''': These courses typically include some reading material (a powerpoint online) and a quiz. To access the classes online, go to MyASU. In the My Classes section, click Blackboard. Click the Courses tab. Enter each class in the search box to find it.<br>
'''Online training''': To register for online training, go [https://cfo.asu.edu/ehs-training here]. Scroll to the bottom and click "Enroll" next to the course you need to take. Make sure to enroll in the refresher course if you've already taken the class. After you enroll, go to blackboard and find the
course listed in your "Courses" module. These courses typically include some reading material (a powerpoint online) and a quiz. Read the material and take the 10-question quiz. These should not take longer than about 30 minutes.<br>




'''REFRESHER COURSES'''<br>
'''REFRESHER COURSES'''<br>
All lab members are required to take annual refresher courses. Reminders will be sent by EHS.
All lab members are required to take annual refresher courses. Reminders will be sent by EHS. Check [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdFV6Qi1CbnEweFhjOHFRa1hMR3NBTWc#gid=0 here] to check your training status. See above for registration instructions under Online training. Email rene dot davis at asu dot edu if the google doc is not accurate or after you complete a training session.


{| {{table}}
{| {{table}}
Line 149: Line 151:
| &#x2611; || ISTB4 || ISTB4 Entrance Group || BHSE
| &#x2611; || ISTB4 || ISTB4 Entrance Group || BHSE
|}
|}
</div>

Revision as of 17:09, 21 September 2013



Lab Duties

Do these tasks yourself or recruit others to help you. Your helpers must have all lab Safety Training completed.


SPRING 2013

Hazardous Waste Behzad Damadzadeh autoclaving biohazard waste, labeling all waste for pick-up, requesting pick-up from EH&S
Tissue Culture Media Prep Dr. Haynes Tissue culture FBS and pen/strep, CO2 incubator water pans
Shipping & Receiving Rene Davis pick up orders from ECG 334A, enter items received in the ordering spreadsheet, check consumables (micropipette tips, serological pipettes, kim wipes, tube racks)
Safety Officer Rene Davis SOP manual, first aid kit, sharps containers, lab-supplied PPE (coats, disposable gloves, autoclave gloves)
Shared Chemicals Behzad Damadzadeh media prep bay tidiness, chemical inventory updates, MSDS collection, fume hood, 37°C shaker-incubator
VOLUNTEER DUTIES Undergraduate volunteers LB agar 100 μg/mL ampicillin plates; LB agar plain plates; Glassware & plasticware; Glass beads; prep and autoclave LB liquid media and 50xTAE; 70% ethanol carboy; 1x TAE carboy


Undergraduate Volunteers

Undergraduate volunteers are invited to learn and carry out media and glassware preparation. Hours for volunteering are on an on-call basis. You will receive a request to work in the lab via a volunteers' mailing list. Volunteers who demonstrate reliability, good technique, and good lab citizenship will be offered opportunities for research projects, either for course credit or a fellowship (e.g., FURI, SOLUR, SynBERC, etc.).

SPRING CYCLE

  • December 1 - January 17: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
  • March 1: Complete ALL required Safety Training by this date. Notify Dr. Haynes via e-mail when all training is completed.
  • March 1: Request ISAAC access to ISTB4. Safety training is required before your ISAAC request is approved.
  • March 1: Attend Haynes lab media prep training.
  • May 2 - spring volunteer cycle ends; continuation through May, June, etc. is welcome.

SUMMER CYCLE

  • May 1 - June 30: requests to join the lab are accepted. Please send requests to karmella dot haynes at asu.
  • July 1 - July 15: complete ALL required Safety Training
  • Mid-July: attend Haynes lab media prep training
  • August 30 - summer volunteer cycle ends; continuation through September, October, etc. is welcome.

FALL CYCLE

  • July 1 - August 31: E-mail Dr. Haynes (karmella dot haynes at asu) to express your interest in volunteering for the lab.
  • September 28: Complete ALL required Safety Training by this date. Notify Dr. Haynes via e-mail when all training is completed.
  • September 28: Request ISAAC access to ISTB4. Safety training is required before your ISAAC request is approved.
  • September 28: Attend Haynes lab media prep training.
  • December 15: Fall volunteer cycle ends; continuation through December, onward is welcome.



Safety Training

INITIAL COURSES
All new lab members and volunteers must take the following courses:

Class Course # In class or Online
1. Laboratory Safety LABSAFE In class
2. Fire Safety FIRE Online
3. Compressed Gas - Under Pressure Online
4. Biosafety & Bloodborne Pathogens Initial Training Online
5. Hazardous Waste Management Online
6. Recombinant DNA Training NIH Online

In class training: Register at the EHS Training Website. Click "Search Course Catalog/Register for Training." Click "Training Registration" on the EH&S Assistant page and follow the instructions. When you attend class, do not forget to sign the instructor's attendance sheet, or your training will not be kept on record.
Registration errors: if you are blocked from registration, contact LMS Support at 480-727-7300 or gtb at asu dot edu.

Online training: To register for online training, go here. Scroll to the bottom and click "Enroll" next to the course you need to take. Make sure to enroll in the refresher course if you've already taken the class. After you enroll, go to blackboard and find the course listed in your "Courses" module. These courses typically include some reading material (a powerpoint online) and a quiz. Read the material and take the 10-question quiz. These should not take longer than about 30 minutes.


REFRESHER COURSES
All lab members are required to take annual refresher courses. Reminders will be sent by EHS. Check here to check your training status. See above for registration instructions under Online training. Email rene dot davis at asu dot edu if the google doc is not accurate or after you complete a training session.

Class Course # In class or Online
1. Laboratory Safety Annual Refresher Training n/a Online
2. Fire Safety & Prevention Annual Refresher Training n/a Online
3. Refresher Biosafety & Bloodborne Pathogens n/a Online
4. Hazardous Waste Management Refresher n/a Online

ISAAC

Request door access online using the secure system. You must have an ASURITE ID and password.

  • Log in at http://isaacrequest.asu.edu/control.php
  • Click the Request link in the left menu
  • Select ISTB4 (Interdisciplinary Science and Technology Building 4)
  • Select the rooms shown in the table below. Access to these doors will allow you to participate in Haynes Lab research & meetings.
  • Read the guidelines and check the "I have read, understood and will comply with these guidelines." box.
  • Click the [Add] button.
  Bulding Room Sub-Location
ISTB4 1st Floor Perimeter
ISTB4 Conference Rooms (BHSE only)
ISTB4 Autoclaves
ISTB4 1st Floor Dock and LER
ISTB4 2nd Floor LER Lab
ISTB4 231 2nd Floor Wet Lab
ISTB4 231 231 Lab/LER/Elev
ISTB4 431 A2 4th Floor Autoclave (BHSE only)
ISTB4 ISTB4 Entrance Group BHSE