Haynes:GOEnrichment

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GOrilla

Intro: These instructions will help you to use the Gene Ontology enRIchment anaLysis and visuaLizAtion tool (GOrilla) to search for enriched GO terms in a target list of genes compared to a background list of genes. The software searches for GO terms that are enriched in the target set compared to the background set using the standard Hyper Geometric statistics.

1. Go to http://cbl-gorilla.cs.technion.ac.il/
2. Set "Choose organism" to the relevant organism (e.g., Homo sapiens = human, Mus musculus = mouse)
3. Set "Choose running mode" to "Two unranked lists of genes (target and background lists)"
4. In the "Target Set" field, paste a list of genes that you want to analyze.
5. For the Background set, upload complete list of all gene symbols for your organism. Use your own or one of the following:
   1. Human genes - GOBg_Human_092014.txt
   2. Mouse genes -