Haynes:GOEnrichment: Difference between revisions
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# Set "Choose organism" to the relevant organism (e.g., Homo sapiens = human, Mus musculus = mouse) | # Set "Choose organism" to the relevant organism (e.g., Homo sapiens = human, Mus musculus = mouse) | ||
# Set "Choose running mode" to "Two unranked lists of genes (target and background lists)" | # Set "Choose running mode" to "Two unranked lists of genes (target and background lists)" | ||
# In the "Target Set" field, paste a list of genes that you want to analyze. | # In the "Target Set" field, paste or upload a list of genes that you want to analyze. txt format, one gene symbol per line, is recommended for the upload option | ||
# For the Background | # For the "Background Set," copy-paste or upload a complete list of all gene symbols for your organism. Use your own or one of the following: | ||
## Human genes - [http://openwetware.org/images/6/6c/GOBg_Human_092014.txt GOBg_Human_092014.txt] | ## Human genes - [http://openwetware.org/images/6/6c/GOBg_Human_092014.txt GOBg_Human_092014.txt] | ||
## Mouse genes - | ## Mouse genes - |
Revision as of 15:51, 11 September 2014
GOrilla
Intro: These instructions will help you to use the Gene Ontology enRIchment anaLysis and visuaLizAtion tool (GOrilla) to search for enriched GO terms in a target list of genes compared to a background list of genes. The software searches for GO terms that are enriched in the target set compared to the background set using the standard Hyper Geometric statistics.
- Go to http://cbl-gorilla.cs.technion.ac.il/
- Set "Choose organism" to the relevant organism (e.g., Homo sapiens = human, Mus musculus = mouse)
- Set "Choose running mode" to "Two unranked lists of genes (target and background lists)"
- In the "Target Set" field, paste or upload a list of genes that you want to analyze. txt format, one gene symbol per line, is recommended for the upload option
- For the "Background Set," copy-paste or upload a complete list of all gene symbols for your organism. Use your own or one of the following:
- Human genes - GOBg_Human_092014.txt
- Mouse genes -