Haynes:Assembly101

Model Procedure for Assembling Parts: Classic Ligation for Beginners
aka Cloning Sensei's guide for the aspiring cloning ninja
by Karmella Haynes

When I was a postdoc in Pam Silver's lab at Harvard (2008 - 2011), my lab mates and I generated large numbers of BioBrick assemblies so rapidly that one of our colleagues in the department referred to us as "cloning ninjas." This guide is based on the original approach developed and recommended by the MIT Registry of Standard Biological parts and has been modified by me to make ligation-based assembly as quick and painless as possible. Let's begin.