Hartings AU Photosynthesis Lab:Materials/Hemoglobin

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Current revision (13:59, 14 January 2013) (view source)
 
Line 29: Line 29:
<u>M33S</u>
<u>M33S</u>
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This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S Asc Hb Col 3).  
+
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in NovaBlue cells in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S Asc Hb Col 3).  
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33S_Sequence | M33S Sequence formatted with amino acids]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33S_Sequence | M33S Sequence formatted with amino acids]]
Line 37: Line 37:
<u>M33S M103S</u>
<u>M33S M103S</u>
-
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S M103S Asc Hb Col 1).  
+
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in Nova Blue cells the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S M103S Asc Hb Col 1).  
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33SM103S_Sequence | M33S/M103S Sequence formatted with amino acids]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33SM103S_Sequence | M33S/M103S Sequence formatted with amino acids]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33SM103S_Sequence_text | M33S/M103S Sequence as text]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33SM103S_Sequence_text | M33S/M103S Sequence as text]]
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 +
<u>M8S M33S M103S</u>
 +
 +
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in NovaBlue cells in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M8S M33S M103S Asc Hb Col 3).
 +
 +
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M8SM33SM103S_Sequence | M8S/M33S/M103S Sequence formatted with amino acids]]
 +
 +
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M8SM33SM103S_Sequence_text | M8S/M33S/M103S Sequence as text]]

Current revision



Hemoglobin Mutants

Wild Type

This DNA was the gift of Daniel Goldberg at WUSL. The original articles for Goldberg's work can be found here and here. The gene is housed in the pET-3d vector, which has ampicillin resistance and no His6 sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb).

WT Sequence formatted with amino acids

WT Sequence as text

K31C

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - K31C Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as K31 Asc Hb).

K31C Sequence formatted with amino acids

K31C Sequence as text

T109C

We have not been able to produce this gene yet using PCR mutation.

K31C T109C

We have not been able to produce this gene yet using PCR mutation.

M33S

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in NovaBlue cells in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S Asc Hb Col 3).

M33S Sequence formatted with amino acids

M33S Sequence as text

M33S M103S

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in Nova Blue cells the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S M103S Asc Hb Col 1).

M33S/M103S Sequence formatted with amino acids

M33S/M103S Sequence as text

M8S M33S M103S

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found as a glycerol stock in NovaBlue cells in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M8S M33S M103S Asc Hb Col 3).

M8S/M33S/M103S Sequence formatted with amino acids

M8S/M33S/M103S Sequence as text

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