Hartings AU Photosynthesis Lab:Materials/Hemoglobin

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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence_text | K31C Sequence as text]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence_text | K31C Sequence as text]]
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<u>T109C</u>
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We have not been able to produce this gene yet using PCR mutation.
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<u>K31C T109C</u>
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We have not been able to produce this gene yet using PCR mutation.
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 +
<u>M33S</u>
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 +
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - M33S Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S Asc Hb).
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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33S_Sequence | M33S Sequence formatted with amino acids]]
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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/M33S_Sequence_text | M33S Sequence as text]]

Revision as of 11:50, 14 January 2013



Hemoglobin Mutants

Wild Type

This DNA was the gift of Daniel Goldberg at WUSL. The original articles for Goldberg's work can be found here and here. The gene is housed in the pET-3d vector, which has ampicillin resistance and no His6 sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb).

WT Sequence formatted with amino acids

WT Sequence as text

K31C

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - K31C Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as K31 Asc Hb).

K31C Sequence formatted with amino acids

K31C Sequence as text

T109C

We have not been able to produce this gene yet using PCR mutation.

K31C T109C

We have not been able to produce this gene yet using PCR mutation.

M33S

This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - M33S Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as M33S Asc Hb).

M33S Sequence formatted with amino acids

M33S Sequence as text

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