Hartings AU Photosynthesis Lab:Materials/Hemoglobin

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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/WT_Sequence_text | WT Sequence as text]]
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/WT_Sequence_text | WT Sequence as text]]
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<u>K31C</u>
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This vector housed in the pET-3d plasmid was prepared via a PCR mutation.
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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence | K31C Sequence formatted with amino acids]]
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[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence_text | K31C Sequence as text]]

Revision as of 11:17, 14 January 2013



Hemoglobin Mutants

Wild Type

This DNA was the gift of Daniel Goldberg at WUSL. The original articles for Goldberg's work can be found here and here. The gene is housed in the pET-3d vector, which has ampicillin resistance and no His6 sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb).

WT Sequence formatted with amino acids

WT Sequence as text

K31C

This vector housed in the pET-3d plasmid was prepared via a PCR mutation.

K31C Sequence formatted with amino acids

K31C Sequence as text

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