Hartings AU Photosynthesis Lab:Materials/Hemoglobin: Difference between revisions
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Hemoglobin Mutants | Hemoglobin Mutants | ||
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/WT_Sequence | WT Sequence]] | <u>Wild Type</u> | ||
This DNA was the gift of [http://dbbs.wustl.edu/faculty/Pages/faculty_bio.aspx?SID=1365 Daniel Goldberg] at WUSL. The original articles for Goldberg's work can be found [http://www.jbc.org/content/268/24/17669.abstract?sid=80e418b5-b56b-4a2f-932f-6b82f3cffe8c here] and [http://www.pnas.org/content/89/24/11696.abstract here]. The gene is housed in the [http://biochem.web.utah.edu/hill/links/pET3a.pdf pET-3d vector], which has ampicillin resistance and no His<sub>6</sub> sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb). | |||
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/WT_Sequence | WT Sequence formatted with amino acids]] | |||
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/WT_Sequence_text | WT Sequence as text]] | |||
Revision as of 08:00, 14 January 2013
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Hemoglobin Mutants
Wild Type
This DNA was the gift of Daniel Goldberg at WUSL. The original articles for Goldberg's work can be found here and here. The gene is housed in the pET-3d vector, which has ampicillin resistance and no His6 sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb).
