HEPES - Revision history

Jakob Suckale at 15:57, 2 November 2011

Jakob Suckale — Wed, 02 Nov 2011 15:57:23 GMT

←Older revision		Revision as of 15:57, 2 November 2011
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-	[[Image:HEPES structure.png\|right\|thumb\|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid)~~;<br>aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9~~]]	+	[[Image:HEPES structure.png\|right\|thumb\|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid)]]

	HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases.		HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases.
-

	== Property of HEPES ==		== Property of HEPES ==

Jakob Suckale at 15:56, 2 November 2011

Jakob Suckale — Wed, 02 Nov 2011 15:56:37 GMT

←Older revision		Revision as of 15:56, 2 November 2011
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	Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.		Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.

-	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)	+	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C); (2nd pK<sub>a</sub> at pH 3 is not of interest)
-	* a second pK<sub>a</sub> at pH 3 is not of interest	+
	* usable buffering range of 6.8 to 8.2		* usable buffering range of 6.8 to 8.2
-	* molecular weight 238.3	+	* molecular weight 238.3 g/mol
	* ΔpK<sub>a</sub>/ΔT = -.014		* ΔpK<sub>a</sub>/ΔT = -.014
	* HEPES contains tertiary amines, which are reactive under certain conditions.		* HEPES contains tertiary amines, which are reactive under certain conditions.
-	* Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S	+	* Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S; N-(2-hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid); aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9

-	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.	+	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.

	== 1M HEPES KOH buffer 7.5 ==		== 1M HEPES KOH buffer 7.5 ==

Jakob Suckale: recipes

Jakob Suckale — Wed, 02 Nov 2011 15:54:45 GMT

recipes

←Older revision		Revision as of 15:54, 2 November 2011
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	HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases.		HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases.
-
-	~~A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].~~


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	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.
		+
		+	== 1M HEPES KOH buffer 7.5 ==
		+	* 700 ml ultrapure water
		+	* 238.3 g HEPES
		+	* KOH (potassium hydroxide) pellets to adjust pH to 7.5
		+	* to 1L w ultrapure water; sterilise by filtration
		+
		+	== 1M HEPES-NaOH pH 7.5 ==
		+	* 700 ml ultrapure water
		+	* 238.3 g HEPES
		+	* ~5.5 g NaOH (sodium hydroxide) pellets to adjust pH to 7.5
		+	* to 1L w ultrapure water; sterilise by filtration

	== Links ==		== Links ==
		+	* original publications: Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292
	* [http://en.wikipedia.org/wiki/Buffer_solution Wikipedia: buffer solution]		* [http://en.wikipedia.org/wiki/Buffer_solution Wikipedia: buffer solution]
	* [http://www.scribd.com/doc/12568914/Common-Buffers-Media-And-Stock-Solutions CP: common buffers]		* [http://www.scribd.com/doc/12568914/Common-Buffers-Media-And-Stock-Solutions CP: common buffers]
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-	[[Category:Material]] ~~and~~ [[Category:Buffers]]	+	[[Category:Material]]
		+	[[Category:Buffers]]

Jakob Suckale: links

Jakob Suckale — Wed, 02 Nov 2011 15:49:22 GMT

links

←Older revision		Revision as of 15:49, 2 November 2011
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	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.
		+
		+	== Links ==
		+	* [http://en.wikipedia.org/wiki/Buffer_solution Wikipedia: buffer solution]
		+	* [http://www.scribd.com/doc/12568914/Common-Buffers-Media-And-Stock-Solutions CP: common buffers]
		+	* [http://www.science.smith.edu/departments/Biochem/Biochem_353/Common_Buffers.html List of common buffers from Smith College]


	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

Jakob Suckale at 15:46, 2 November 2011

Jakob Suckale — Wed, 02 Nov 2011 15:46:11 GMT

←Older revision		Revision as of 15:46, 2 November 2011
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	[[Image:HEPES structure.png\|right\|thumb\|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid);<br>aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9]]		[[Image:HEPES structure.png\|right\|thumb\|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid);<br>aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9]]
		+
		+	HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases.

	A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].		A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].
		+
		+
		+	== Property of HEPES ==

	Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.		Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.
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	* ΔpK<sub>a</sub>/ΔT = -.014		* ΔpK<sub>a</sub>/ΔT = -.014
	* HEPES contains tertiary amines, which are reactive under certain conditions.		* HEPES contains tertiary amines, which are reactive under certain conditions.
-	* See the structure of [http://store.p212121.com/products/HEPES-500-g.html HEPES].
	* Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S		* Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S

Jakob Suckale: illustration

Jakob Suckale — Wed, 02 Nov 2011 15:36:01 GMT

illustration

←Older revision		Revision as of 15:36, 2 November 2011
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-	N-(2-~~hydroxyethyl~~)~~piperazine~~-N’-(2-~~ethanesulfonic~~ acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS ~~NUMBER~~: 7365-45-9	+	[[Image:HEPES structure.png\|right\|thumb\|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid);<br>aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9]]

-	A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.	+	A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].
		+
		+	Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.

	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)		* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)

Jakob Suckale: references

Jakob Suckale — Wed, 02 Nov 2011 15:30:10 GMT

references

←Older revision		Revision as of 15:30, 2 November 2011
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	N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9		N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9

-	A Good buffer ~~<cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>~~. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.	+	A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.

	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)		* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)
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	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.
-
-	~~<biblio>~~
-	~~# Good66 pmid=5942950~~
-	~~# Good74 pmid=4206745~~
-	~~# Blanchard84 pmid=6717292~~
-	~~</biblio>~~


	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

Eric Hanson at 04:19, 31 July 2010

Eric Hanson — Sat, 31 Jul 2010 04:19:42 GMT

←Older revision		Revision as of 04:19, 31 July 2010
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	N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9		N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9

-	A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.	+	A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.

	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)		* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)
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	* molecular weight 238.3		* molecular weight 238.3
	* ΔpK<sub>a</sub>/ΔT = -.014		* ΔpK<sub>a</sub>/ΔT = -.014
-	*HEPES contains tertiary amines, which are reactive under certain conditions. See ~~its~~ structure ~~in the~~ [http://~~www~~.~~sigmaaldrich~~.com/~~catalog~~/~~search/ProductDetail/SIGMA/H3375 Sigma catalog~~].	+	* HEPES contains tertiary amines, which are reactive under certain conditions.
-	*Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S	+	* See the structure of [http://store.p212121.com/products/HEPES-500-g.html HEPES].
		+	* Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S

	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.

Torsten Waldminghaus: added tag

Torsten Waldminghaus — Tue, 11 Mar 2008 21:31:50 GMT

added tag

←Older revision		Revision as of 21:31, 11 March 2008
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	# Blanchard84 pmid=6717292		# Blanchard84 pmid=6717292
	</biblio>		</biblio>
		+
		+
		+	[[Category:Material]] and [[Category:Buffers]]

Kathmc at 15:52, 5 May 2006

Kathmc — Fri, 05 May 2006 15:52:36 GMT

←Older revision		Revision as of 15:52, 5 May 2006
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	N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9		N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9

-	A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range.	+	A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.

	* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)		* pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)
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	* molecular weight 238.3		* molecular weight 238.3
	* ΔpK<sub>a</sub>/ΔT = -.014		* ΔpK<sub>a</sub>/ΔT = -.014
		+	*HEPES contains tertiary amines, which are reactive under certain conditions. See its structure in the [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/H3375 Sigma catalog].
		+	*Chemical formula: C<sub>8</sub>H<sub>18</sub>N<sub>2</sub>O<sub>4</sub>S

	Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.