HEPES
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A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range. | A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range. | ||
| - | * pK<sub>a</sub> at 25C of 7.55 (a second | + | * pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C) |
| + | * a second pK<sub>a</sub> at pH 3 is not of interest | ||
* usable buffering range of 6.8 to 8.2 | * usable buffering range of 6.8 to 8.2 | ||
* molecular weight 238.3 | * molecular weight 238.3 | ||
Revision as of 17:59, 14 April 2006
N-(2-hydroxyethyl)piperazine-Nā-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9
A Good buffer [1][2][3]. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range.
- pKa at 25C of 7.55 (7.31 at 37C)
- a second pKa at pH 3 is not of interest
- usable buffering range of 6.8 to 8.2
- molecular weight 238.3
- ΔpKa/ΔT = -.014
Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized.
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. . pmid:5942950.
- Good NE and Izawa S. . pmid:4206745.
- Blanchard JS. . pmid:6717292.
