Guojia:CV

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Jia Guo, Ph.D.

Stanford University

Stanford, CA 94305

Email: jiaguo@stanford.edu

Education

2009, PhD, Columbia University (Dissertation with Distinction)

2005, BS, University of Science and Technology of China (Graduation with Honor)

Honors and Awards

2011 Young Investigator Scholarship (Alzheimer’s Drug Discovery Foundation)

2009 Dissertation with Distinction (Columbia University)

2009 Pegram Award for Outstanding Graduate Work (Columbia University)

2005-2009 Faculty Fellowship (Columbia University)

2005 Graduation with Honor (University of Science and Technology of China)

2001-2005 Outstanding Student Scholarship (University of Science and Technology of China)

2004 University Excellent Project (University of Science and Technology of China)

Research Experience

  • Stanford University (2010-presesent)
    • The novel Comet-FISH to study genome integrity and epigenetics.
      • Designed and synthesized novel probes for Comet-FISH
      • Utilized the developed Comet-FISH to study oxidative DNA damage


    • Real-time multiplexed cellular imaging with bioconjugated polyfluorophores
      • Designed the strategy of multiplexed cellular imaging with polyfluorophores
      • Synthesized polyfluorophores on a DNA backbone
      • Synthesized polyfluorophore-antibody conjugates
      • Produced the multiplexed cellular imaging data


    • Polyfluorophore-peptide conjugates as real-time caspase sensors
      • Designed the molecular approach of real-time caspase sensing
      • Synthesized Polyfluorophore-peptide conjugates
      • Monitored caspase activity in live cells in real-time


  • Columbia University (2005-2009)
    • High throughput DNA sequencing by synthesis (SBS) with novel nucleotide analogues
      • Designed the DNA sequencing strategy with hybrid Sanger and SBS
      • Designed and synthesized 3’-O-modified nucleotide reversible terminators
      • Designed and synthesized chemically cleavable fluorescent dideoxynucleotides
      • Produced four-color DNA sequencing data on a DNA chip


    • Binary probes to monitor mRNA in live cells
      • Designed the approach of using binary probes to study mRNA
      • Designed and synthesized fluorescent binary probes
      • Demonstrated the application of binary probes to detect mRNA


    • Biotinylated dideoxynucleotides for DNA sequencing by MALDI-TOF MS
      • Designed the DNA sequencing strategy with biotinylated dideoxynucleotides
      • Designed and synthesized biotinylated dideoxynucleotides
      • Studied mitochondrial SNP genotyping with this novel approach


  • University of Science and Technology of China (2003-2005)
    • Development of self-clean glasses
      • Prepared and characterized SiO2, ZnO thin film


  • Chinese Academe of Science (Summer 2004)
    • DNA separation by capillary electrophoresis
      • Optimized the conditions for efficient DNA separation


Teaching Experience

  • Columbia University
    • Teaching Assistant of General Chemistry Lecture (2006-2007)
      • Taught weekly recitation to supplement lecture courses
      • Evaluated students’ ability to solve problems in general chemistry


    • Teaching Assistant of Organic Chemistry Lab (2005-2006)
      • Delivered weekly lectures
      • Instructed students in theory and procedure

Publication

1. J. Guo, J. Ju and N. J. Turro. "Fluorescent hybridization probes for nucleic acid detection" Anal Bioanal Chem, 2012, 402:3115-3125.

2. S. Wang, J. Guo, T. Ono and E. T. Kool. "DNA Polyfluorophores for Real-Time Multicolor Tracking of Dynamic Biological Systems" Angew Chem Int Ed, 2012, in press.

3. C. Qiu, S. Kumar, J. Guo, J. Lu, S. Shi, S. Kalachikov, J. Russo, A. Naini, E. Schon and J. Ju. “Mitochondrial SNP Genotyping by MALDI-TOF Mass Spectrometry Using Cleavable Biotinylated Dideoxynucleotides” Anal Biochem, 2012, in press.

4. C. Qiu, S. Kumar, J. Guo, L. Yu, W. Guo, S. Shi, J. Russo and J. Ju. “Design and Synthesis of Cleavable Biotinylated Dideoxynucleotides for DNA Sequencing by MALDI-TOF Mass Spectrometry” Anal Biochem, 2012, in press.

5. J. Guo, S. Wang, N. Dai, Y. N. Teo and E. T. Kool. “Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging” PNAS, 2011, 108: 3493-3498. (highlighted in Nat Meth)

6. N. Dai, J. Guo, Y. N. Teo and E. T. Kool. “Protease Probes Built from DNA: Multispectral Fluorescent DNA–peptide Conjugates as Caspase Sensors” Angew Chem Int Ed, 2011, 50:5105-5109. (highlighted as hot paper)

7. J. Guo, L. Yu, N. J. Turro and J. Ju. “An integrated system for DNA sequencing by synthesis using novel nucleotide analogues” Acc Chem Res, 2010, 43: 551-563.

8. L. Yu, J. Guo, N. Xu, Z. Li and J. Ju. “DNA Sequencing by Synthesis Using Novel Nucleotide Analogues" The Handbook of Plant Mutation Screening, 2010, 319-336.

9. J. Guo, N. Xu, Z. Li, S. Zhang, J. Wu, D. H. Kim, M. S. Marma, Q. Meng, H. Cao, X. Li, S. Shi, L. Yu, S. Kalachikov, J. Russo, N. J. Turro and J. Ju. “Four-color DNA sequencing with 3’-O-modified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides” PNAS, 2008, 105: 9145-9150. (highlighted in PNAS, ranked 50 most read PNAS papers)

10. Z. Wang, X. Xu, Y. Chen, S. Zhou, N. Kong, J. Guo, H. Liu, C. Shi. “Characteristics of the ZnxMg1-xO films grown by electrophoresis method” Acta Physica Sinica, 2004, 53: 3924-3928.

11. J. Guo, M. Gong, X. Xu, W. Zhang, H. Guo, L. Wang, Y. Chen, P. Liu, H. Liu. “The influence of anneal on the properties of SiO2 thin films prepared by sol-gel method” J Funct Mater, 2004, 35: 3171-3174.

Patents

1. J. Ju, D. H. Kim, J. Guo, Q. Meng, Z. Li and H. Cao. “DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators” PCT Int. Appl. 2009, WO 2009054922.

2. J. Ju, H. Cao, Z. Li, Q. Meng, J. Guo, S. Zhang and L. Yu. “Design and synthesis of labile azido linkers for conjugation of fluorescent dyes to nucleotides for use as reversible terminators in DNA sequencing by synthesis” PCT Int. Appl. 2009, WO 2009051807.

Presentations

1. J. Guo, S. Wang, N. Dai, Y. N. Teo, E. T. Kool. “Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging” Poster Presentation at the 5th International Meeting on Synthetic Biology, Stanford, CA, June 15-17, 2011.

2. J. Guo, N. Xu, Z. Li, S. Zhang, J. Wu, D. H. Kim, M. S. Marma, Q. Meng, H. Cao, X. Li, S. Shi, L. Yu, S. Kalachikov, J. Russo, N. J. Turro and J. Ju. “Four-color DNA sequencing with 3’-O-modified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides” Poster Presentation at the 238th ACS National Meeting, Washington, DC, August 16-20, 2009.

3. J. Guo, N. J. Turro and J. Ju. “Toward the $1000 genome: molecular engineering approaches for DNA sequencing by synthesis” Presentation at Columbia Chemistry/Biology Interface Workshop, New York, NY, February 24, 2009.

4. J. Guo, N. Xu, N. J. Turro and J. Ju. “Design and synthesis of labile azido linkers for conjugation of fluorescent dyes to nucleotides for use as reversible terminators in DNA sequencing by synthesis” Poster Presentation at the Symposium on Newer Trends in Photochemistry, New York, NY, May 23, 2008.

5. J. Guo, N. J. Turro and J. Ju. “High throughput four-color DNA sequencing by synthesis on a chip” Presentation at the Wyeth/Columbia Research Workshop, New York, NY, May 6, 2008.

6. J. Guo, N. Xu, N. J. Turro and J. Ju. “Four-color DNA sequencing with hybrid Sanger and SBS approach” Poster Presentation at the NHGRI Sequencing Technology Conference, San Diego, CA, March 18, 2008.

7. J. Guo, N. J. Turro, J. Ju. “Fluorescent cDNA Labeling with Click Chemistry for DNA Microarray Analysis” Poster Presentation at the International Conference on Genomics, Hangzhou, China, October 25, 2006.

Last Updated: June, 2012


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