GroseLab:Protocols
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Revision as of 15:03, 12 March 2011 by
Julianne H. Grose
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GroseLab
Contents
1
Recipes
2
Basic protocols
2.1
DNA
2.2
Protein
3
Bacterial Protocols
3.1
DNA
3.2
Protein
4
Yeast Protocols
4.1
DNA
4.2
Protein
Recipes
Common buffers
Common media
Stock solutions
Basic protocols
DNA
TAQ PCR
Phusion PCR
Cloning
Protein
Western Blot
Bacterial Protocols
DNA
DH5α Competent Cells
Electrocompetent cells
Linear transformation
Protein
HIS tag purification
GST purification
Myc purification
FLAG purification
Yeast Protocols
DNA
Competent Cells and Transformation
Quick Genomic DNA prep
Yeast Plasmid Prep
Protein
IP kinase assays
in vivo PASK phosphoryation assay
in vivo Ugp1 phosphorylation assay
his tag purification
GST purification
Myc purification
FLAG purification
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