GroseLab:Protocols: Difference between revisions
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==Recipes== | ==Recipes== | ||
* [[ | * [[Common buffers|Common buffers]] | ||
* [[ | * [[Common media|Common media]] | ||
* [[ | * [[Stock solutions|Stock solutions]] | ||
==Basic protocols== | ==Basic protocols== | ||
===DNA=== | ===DNA=== | ||
* [[ | * [[TAQ PCR]] | ||
* [[ | * [[Phusion PCR]] | ||
* [[ | * [[Cloning]] | ||
===Protein=== | ===Protein=== | ||
* [[ | * [[Western Blot]] | ||
==Bacterial Protocols== | ==Bacterial Protocols== | ||
===DNA=== | ===DNA=== | ||
*[[ | *[[DH5α Competent Cells]] | ||
*[[ | *[[Electrocompetent cells]] | ||
*[[ | *[[Linear transformation]] | ||
===Protein=== | ===Protein=== | ||
*[[HIS tag purification]] | |||
*[[GST purification]] | |||
*[[Myc purification]] | |||
*[[FLAG purification]] | |||
==Yeast Protocols== | |||
== | |||
===DNA=== | ===DNA=== | ||
*[[ | *[[Competent Cells and Transformation]] | ||
*[[ | *[[Quick Genomic DNA prep]] | ||
*[[ | *[[Yeast Plasmid Prep]] | ||
===Protein=== | ===Protein=== | ||
*[[IP kinase assays]] | |||
*[[ | *[[in vivo PASK phosphoryation assay]] | ||
*[[ | *[[in vivo Ugp1 phosphorylation assay]] | ||
*[[ | *[[his tag purification]] | ||
*[[ | *[[GST purification]] | ||
*[[ | *[[Myc purification]] | ||
*[[ | *[[FLAG purification]] | ||
*[[ | |||
Revision as of 15:03, 12 March 2011
