GroseLab:Protocols: Difference between revisions

Revision as of 15:03, 12 March 2011

@@ Line 3: / Line 3: @@
 [[Image:Diverse_e_Coli.png|400px]] [[Image:S-cerevisiaecolored.JPG]]
-=Universal Protocols=
+==Recipes==
+* [[Common buffers|Common buffers]]
+* [[Common media|Common media]]
+* [[Stock solutions|Stock solutions]]
-[[Buffer Prep]]
+==Basic protocols==
+===DNA===
+* [[TAQ PCR]]
+* [[Phusion PCR]]
+* [[Cloning]]
-[[Native Gel]]
+===Protein===
+* [[Western Blot]]
-[[Phusion PCR]]
+==Bacterial Protocols==
+===DNA===
+*[[DH5α Competent Cells]]
+*[[Electrocompetent cells]]
+*[[Linear transformation]]
-=Yeast protocols=
+===Protein===
+*[[HIS tag purification]]
+*[[GST purification]]
+*[[Myc purification]]
+*[[FLAG purification]]
-[[IP KINASE ASSAY]]
+==Yeast Protocols==
+===DNA===
+*[[Competent Cells and Transformation]]
+*[[Quick Genomic DNA prep]]
+*[[Yeast Plasmid Prep]]
-==UGP1 Purification==
+===Protein===
+*[[IP kinase assays]]
-=Bacterial Protocols=
+*[[in vivo PASK phosphoryation assay]]
+*[[in vivo Ugp1 phosphorylation assay]]
-[[DH5α Competent Cells]]
+*[[his tag purification]]
+*[[GST purification]]
-[[Eleanor Electrocompetency for e. Coli]]
+*[[Myc purification]]
+*[[FLAG purification]]
-[[Reading Oxygen Consumption Plates]]
-[[Cloning Protocol]]