GroseLab:Protocols: Difference between revisions
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[[Image:Diverse_e_Coli.png|400px]] [[Image:S-cerevisiaecolored.JPG]] | [[Image:Diverse_e_Coli.png|400px]] [[Image:S-cerevisiaecolored.JPG]] | ||
= | ==Recipes== | ||
* [[Common buffers|Common buffers]] | |||
* [[Common media|Common media]] | |||
* [[Stock solutions|Stock solutions]] | |||
= | ==Basic protocols== | ||
===DNA=== | |||
* [[TAQ PCR]] | |||
* [[Phusion PCR]] | |||
* [[Cloning]] | |||
== | ===Protein=== | ||
* [[Western Blot]] | |||
=Bacterial Protocols= | ==Bacterial Protocols== | ||
===DNA=== | |||
*[[DH5α Competent Cells]] | |||
*[[Electrocompetent cells]] | |||
*[[Linear transformation]] | |||
[[ | ===Protein=== | ||
*[[HIS tag purification]] | |||
*[[GST purification]] | |||
*[[Myc purification]] | |||
*[[FLAG purification]] | |||
[[ | ==Yeast Protocols== | ||
===DNA=== | |||
*[[Competent Cells and Transformation]] | |||
*[[Quick Genomic DNA prep]] | |||
*[[Yeast Plasmid Prep]] | |||
[[ | ===Protein=== | ||
*[[IP kinase assays]] | |||
*[[in vivo PASK phosphoryation assay]] | |||
*[[in vivo Ugp1 phosphorylation assay]] | |||
*[[his tag purification]] | |||
*[[GST purification]] | |||
*[[Myc purification]] | |||
*[[FLAG purification]] | |||
Revision as of 15:03, 12 March 2011
