Griffitts:Common media: Difference between revisions
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==Bacterial Media== | ==Bacterial Media and Additives== | ||
===[[Wikipedia:Lysogeny broth|LB broth]] (1 L)=== | ===[[Wikipedia:Lysogeny broth|LB broth]] (1 L)=== | ||
* 1 L dH<sub>2</sub>0 | * 1 L dH<sub>2</sub>0 | ||
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Filter sterilize | Filter sterilize | ||
Note: mannitol and lactose are soluble at 30% only upon heating | Note: mannitol and lactose are soluble at 30% only upon heating | ||
==Plant Media== | ==Plant Media== | ||
Revision as of 15:04, 7 September 2007
Bacterial Media and Additives
LB broth (1 L)
- 1 L dH20
- 10 g Bacto tryptone
- 5 g Bacto yeast extract
- 5 g NaCl
- 1 mL 2N NaOH
- 12 g agar (optional)
LB-Top broth (500 mL)
- 500 mL dH20
- 5 g Bacto tryptone
- 2.5 g Bacto yeast extract
- 2.5 g NaCl
- 0.5 mL 2N NaOH
- 2 g agar (optional)
Autoclave Add 2.5 mM CaCl2 before phage infections
TY broth (1 L)
- 1 L dH20
- 6 g Bacto tryptone
- 3 g Bacto yeast extract
- 0.5 g CaCl2·2H20
- 12 g Bacto agar (optional)
Autoclave Note: for motility assays, mage agar 0.24%
Standard Minimal Medium (SMM)(250 mL)
To 250 mL sterile dH20 or water agar add:
- 2.5 mL 100X SMAJ (see below)
- 2.5 mL 100X MCBT (see below)
- 2.5 mL 30% carbon source
- antibiotics (optional)
100X SMAJ (50 mL)
- ~30 mL ddH20
- 8 g KH2PO4
- 12 g K2HPO4
- 2 g NaCl
- 4 g NH4Cl
(takes a while to dissolve)
Filter sterilize
100X MCBT (50 mL)
Filter sterilize Note: Biotin takes a while to dissolve
100X (30%) carbon source (50 mL)
- 15 g carbon source (e.g. sucrose, mannitol, glucose, lactose, sodium succinate).
- QS to volume with ddH20
Filter sterilize Note: mannitol and lactose are soluble at 30% only upon heating
