http://openwetware.org/index.php?title=Griffin:Antibody_Elution_Buffers&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Thu, 23 May 2013 17:30:51 GMT http://openwetware.org/index.php?title=Griffin:Antibody_Elution_Buffers&diff=244401&oldid=prev <p>New page: Elution buffers are essential in order to affinity purify antibodies. There are several out there, and below are a representation of 4 very good ones. *'''100mM Glycine, pH2.5 (acidic pH)...</p> <p><b>New page</b></p><div>Elution buffers are essential in order to affinity purify antibodies. There are several out there, and below are a representation of 4 very good ones.<br /> <br /> *'''100mM Glycine, pH2.5 (acidic pH)'''<br /> <br /> *'''1M Triethanolamine; TEA (basic pH)'''<br /> <br /> *'''4M MgCl2 (high salt)'''<br /> <br /> *'''1M NaCl/PBS (high salt)'''<br /> <br /> In general high salt concentration or extreme pH will disrupt the antibody-protein binding. The optimal elution buffer to use will have to be<br /> determined experimentally by the user.<br /> <br /> [[Category:Protocol]] [[Category:In vitro]] [[Category:Protein]]</div> Fri, 19 Sep 2008 19:14:20 GMT Korey Griffin http://openwetware.org/wiki/Talk:Griffin:Antibody_Elution_Buffers