Goat PTBP1 antibody (EB06088): Difference between revisions
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According to the Everest product page the antibody worked in a paraffin sections of human skin [http://everestbiotech.com/product/goat-anti-ptbp1-ptb-antibody/] and was used in Galban'08 (PMID 17967866, [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223304/?tool=pubmed]). This study also shows [[protein blot]]s using the antibody in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223304/figure/f6/ figure 6]. | According to the Everest product page the antibody worked in a paraffin sections of human skin [http://everestbiotech.com/product/goat-anti-ptbp1-ptb-antibody/] and was used in Galban'08 (PMID 17967866, [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223304/?tool=pubmed]). This study also shows [[protein blot]]s using the antibody in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223304/figure/f6/ figure 6]. | ||
[[Image:PTBP1 goat antibody Everest vs Zymed INS1 PFA fixed GSDB.jpg|500px]] | |||
Antibody labelling of the same batch of INS1 cell line with the Everest goat antibody (middle) showed no specific staining beyond the unspecific fluorescence also observed in the no primary antibody control (right). Another antibody for PTBP1 from Zymed now sold by Invitrogen shows the typical nuclear labelling of PTBP1 in this cell line. [[User:Jakob Suckale|- JS]] | |||
Latest revision as of 02:48, 13 August 2010
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| Antibody Type | goat polyclonal |
| Target UniProt ID | P26599 |
| Cross-Reactive w | mouse, rat |
| Raised Against | N-terminal peptide DGIVPDIAVGTKR |
| Provider | Everest Biotech |
| Suitability for.. | |
| Western Blot | worked |
| Staining fixed cells | failed |
| Staining cryo-sections | |
| Staining paraffin sections | worked |
| Help | |
Duplicates
- Everest Biotech goat Anti-PTBP1 / PTB Antibody (EB06088) $300/100µg (2010/7)
- abcam goat PTBP1 antibody (ab5642) $320/100µg (2010/7)
- Sigma goat PTBP1/PTB antibody (SAB2500834) $300/100µg (2010/7)
All pages above show the same protein blot. Everest is the original producer according to a statement on their page. abcam and Sigma are re-sellers not referencing the source of the product.
Experimental data
According to the Everest product page the antibody worked in a paraffin sections of human skin [1] and was used in Galban'08 (PMID 17967866, [2]). This study also shows protein blots using the antibody in figure 6.
Antibody labelling of the same batch of INS1 cell line with the Everest goat antibody (middle) showed no specific staining beyond the unspecific fluorescence also observed in the no primary antibody control (right). Another antibody for PTBP1 from Zymed now sold by Invitrogen shows the typical nuclear labelling of PTBP1 in this cell line. - JS
