Glycerol - Revision history

Jakob Suckale: categories

2009-11-11T14:49:16Z

Jakob Suckale at 14:48, 11 November 2009

2009-11-11T14:48:37Z

←Older revision		Revision as of 14:48, 11 November 2009
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	== Uses of glycerol ==		== Uses of glycerol ==
-	* Glycerol is used in many loading buffers to '''increase the density''' of the solution which enables good loading into already liquid filled wells of agarose and polyacrylamide gels. See [[agarose gel loading buffer]] and [[SDS sample buffer]].	+	=== Density ===
		+	Glycerol is used in many loading buffers to '''increase the density''' of the solution which enables good loading into already liquid filled wells of agarose and polyacrylamide gels. See [[agarose gel loading buffer]] and [[SDS sample buffer]].

-	* Glycerol is also used to '''decrease the freezing point''' of protein solution which are easily degraded by repeated freeze/thaw cycles. A 50% (w/v) glycerol/water solution freezes at -25ºC and can thus be stored in a -20ºC freezer. Note that glycerol with 1.26g/cm<sup>3</sup> is much denser than water and, therefore, a 50% (v/v) solution will be very different from a 50% (w/v) solution. For example, [[restriction enzymes]] are typically stored in a glycerol/water solution	+	=== Freezing ===
		+	Glycerol is also used to '''decrease the freezing point''' of protein solution which are easily degraded by repeated freeze/thaw cycles. A 50% (w/v) glycerol/water solution freezes at -25ºC and can thus be stored in a -20ºC freezer. Note that glycerol with 1.26g/cm<sup>3</sup> is much denser than water and, therefore, a 50% (v/v) solution will be very different from a 50% (w/v) solution. For example, [[restriction enzymes]] are typically stored in a glycerol/water solution.

	== Glycerol solution recipes ==		== Glycerol solution recipes ==

Jakob Suckale: uses of glycerol

2009-11-11T14:47:21Z

uses of glycerol

←Older revision		Revision as of 14:47, 11 November 2009
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	* Glycerol is used in many loading buffers to '''increase the density''' of the solution which enables good loading into already liquid filled wells of agarose and polyacrylamide gels. See [[agarose gel loading buffer]] and [[SDS sample buffer]].		* Glycerol is used in many loading buffers to '''increase the density''' of the solution which enables good loading into already liquid filled wells of agarose and polyacrylamide gels. See [[agarose gel loading buffer]] and [[SDS sample buffer]].

-		+	* Glycerol is also used to '''decrease the freezing point''' of protein solution which are easily degraded by repeated freeze/thaw cycles. A 50% (w/v) glycerol/water solution freezes at -25ºC and can thus be stored in a -20ºC freezer. Note that glycerol with 1.26g/cm<sup>3</sup> is much denser than water and, therefore, a 50% (v/v) solution will be very different from a 50% (w/v) solution. For example, [[restriction enzymes]] are typically stored in a glycerol/water solution

	== Glycerol solution recipes ==		== Glycerol solution recipes ==

Jakob Suckale: uses of glycerol

2009-11-11T14:47:04Z

uses of glycerol

←Older revision		Revision as of 14:47, 11 November 2009
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	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to as glycerine or glycerin.		'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to as glycerine or glycerin.

-	== ~~Recipes~~ ==	+	== Uses of glycerol ==
		+	* Glycerol is used in many loading buffers to '''increase the density''' of the solution which enables good loading into already liquid filled wells of agarose and polyacrylamide gels. See [[agarose gel loading buffer]] and [[SDS sample buffer]].
		+
		+
		+
		+	== Glycerol solution recipes ==
	* [[Silver: 50% Glycerol\|50% glycerol solution recipe from the Silver lab]]		* [[Silver: 50% Glycerol\|50% glycerol solution recipe from the Silver lab]]
	* [[Griffitts:Stock_solutions#80.25_Glycerol_.2875_mL.29\|80% glycerol solution recipe from the Griffitts lab]]		* [[Griffitts:Stock_solutions#80.25_Glycerol_.2875_mL.29\|80% glycerol solution recipe from the Griffitts lab]]

Jakob Suckale: recipe

2009-11-11T14:35:12Z

recipe

←Older revision		Revision as of 14:35, 11 November 2009
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	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to as glycerine or glycerin.		'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to as glycerine or glycerin.
		+
		+	== Recipes ==
		+	* [[Silver: 50% Glycerol\|50% glycerol solution recipe from the Silver lab]]
		+	* [[Griffitts:Stock_solutions#80.25_Glycerol_.2875_mL.29\|80% glycerol solution recipe from the Griffitts lab]]

	== See also ==		== See also ==
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	* [http://www.bio.net/bionet/mm/methods/1996-August/048145.html Discussion on the use of glycerol to preserve proteins at bio.net]		* [http://www.bio.net/bionet/mm/methods/1996-August/048145.html Discussion on the use of glycerol to preserve proteins at bio.net]
	* [http://www.dow.com/glycerine/resources/freezept.htm Freezing point of glycerol/water solution]		* [http://www.dow.com/glycerine/resources/freezept.htm Freezing point of glycerol/water solution]
-
-	~~=== Recipes ===~~
-	* [[Silver: 50% Glycerol\|50% glycerol solution recipe from the Silver lab]]
-	* [[Griffitts:Stock_solutions#80.25_Glycerol_.2875_mL.29\|80% glycerol solution recipe from the Griffitts lab]]\

Jakob Suckale: see also

2009-11-11T14:34:48Z

Jakob Suckale at 14:31, 11 November 2009

2009-11-11T14:31:07Z

←Older revision		Revision as of 14:31, 11 November 2009
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	[[Image:Glycerol chemical formula.svg\|thumb\|right\|Chemical structure of '''glycerol''', also known as glycerine and propane-1,2,3-triol]]		[[Image:Glycerol chemical formula.svg\|thumb\|right\|Chemical structure of '''glycerol''', also known as glycerine and propane-1,2,3-triol]]

-	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids.	+	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to glycerine or glycerin.

Jakob Suckale: illustration

2009-11-11T14:27:14Z

illustration

←Older revision		Revision as of 14:27, 11 November 2009
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		+	[[Image:Glycerol chemical formula.svg\|thumb\|right\|Chemical structure of '''glycerol''', also known as glycerine and propane-1,2,3-triol]]
		+
	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids.		'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids.

Jakob Suckale: * page created *

2009-11-11T14:25:39Z

*** page created ***

New page

'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids.

←Older revision		Revision as of 14:49, 11 November 2009
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	* [http://www.bio.net/bionet/mm/methods/1996-August/048145.html Discussion on the use of glycerol to preserve proteins at bio.net]		* [http://www.bio.net/bionet/mm/methods/1996-August/048145.html Discussion on the use of glycerol to preserve proteins at bio.net]
	* [http://www.dow.com/glycerine/resources/freezept.htm Freezing point of glycerol/water solution]		* [http://www.dow.com/glycerine/resources/freezept.htm Freezing point of glycerol/water solution]
		+
		+	[[Category:Material]]
		+	[[Category:Chemical]]

←Older revision		Revision as of 14:34, 11 November 2009
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	[[Image:Glycerol chemical formula.svg\|thumb\|right\|Chemical structure of '''glycerol''', also known as glycerine and propane-1,2,3-triol]]		[[Image:Glycerol chemical formula.svg\|thumb\|right\|Chemical structure of '''glycerol''', also known as glycerine and propane-1,2,3-triol]]

-	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to glycerine or glycerin.	+	'''Glycerol''' is an alcohol often used in the lab to avoid freezing and increase the density of liquids. Its standardised IUPAC name is propane-1,2,3-triol. It is also often referred to as glycerine or glycerin.
		+
		+	== See also ==
		+	* [http://en.wikipedia.org/wiki/Glycerine Glycerol at the Wikipedia]
		+	* [http://www.bio.net/bionet/mm/methods/1996-August/048145.html Discussion on the use of glycerol to preserve proteins at bio.net]
		+	* [http://www.dow.com/glycerine/resources/freezept.htm Freezing point of glycerol/water solution]
		+
		+	=== Recipes ===
		+	* [[Silver: 50% Glycerol\|50% glycerol solution recipe from the Silver lab]]
		+	* [[Griffitts:Stock_solutions#80.25_Glycerol_.2875_mL.29\|80% glycerol solution recipe from the Griffitts lab]]\

Glycerol - Revision history

Jakob Suckale: categories

Jakob Suckale at 14:48, 11 November 2009

Jakob Suckale: uses of glycerol

Jakob Suckale: uses of glycerol

Jakob Suckale: recipe

Jakob Suckale: see also

Jakob Suckale at 14:31, 11 November 2009

Jakob Suckale: illustration

Jakob Suckale: *** page created ***

Jakob Suckale: * page created *