Glutaraldehyde (Pentanedial): Difference between revisions
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== Uses == | == Uses == | ||
* Glutaraldehyde is used to fix specimen before [[electron microscopy]] where it is employed alone or mixed with [[polymethanal (paraformaldehyde)]] as the first of 2 fixations followed by osmium tetroxide. | |||
* Glutaraldehyde is used as an amine cross-linker. | |||
* Glutaraldehyde is used in [[SDS-PAGE]] to fix/crosslink proteins and peptides prior to staining. Gels are treated with a 5% solution for ~30 min, after which it must be thoroughly washed to remove the yellow stain brought about by reacting with free [[Tris]]. Alternatively, gels can be washed before fixation. | |||
== Recipes == | == Recipes == | ||
Revision as of 04:57, 3 November 2011
Glutaraldehyde (1,5-pentanedial) is a common fixative in biology. It is used to reduce degradation in cells, tissues, and entire organisms before further experiments like electron microscopy.
Properties
Uses
- Glutaraldehyde is used to fix specimen before electron microscopy where it is employed alone or mixed with polymethanal (paraformaldehyde) as the first of 2 fixations followed by osmium tetroxide.
- Glutaraldehyde is used as an amine cross-linker.
- Glutaraldehyde is used in SDS-PAGE to fix/crosslink proteins and peptides prior to staining. Gels are treated with a 5% solution for ~30 min, after which it must be thoroughly washed to remove the yellow stain brought about by reacting with free Tris. Alternatively, gels can be washed before fixation.
