Gill:Vogel-Bonner glucose (VBG) minimal medium

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Materials

100 mL 500 mL
50X VBG salts 2 ml 10 ml
20% glucose 5 ml 25 ml
Noble agar 2 g 10 g
dH2O 93 ml 465 ml

Procedure

Add agar to water, autoclave, cool slightly (about 5-10 min at RT); pre-warm 50X VBG salts to 50 – 55 ºC. While gently mixing, slowly aseptically add VBG salts and glucose, mix and pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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