Gill:Vogel-Bonner glucose (VBG) minimal medium: Difference between revisions
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(New page: ==Materials== {| class="wikitable" |- ! ! 100 mL ! 500 mL |- | 50X VBG salts | 2 ml | 10 ml |- | 20% glucose | 5 ml | 25 ml |- | Noble agar | 2 g | 10 g |- | dH<sub>2</sub...) |
(No difference)
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Latest revision as of 13:38, 23 April 2013
Materials
| 100 mL | 500 mL | |
|---|---|---|
| 50X VBG salts | 2 ml | 10 ml |
| 20% glucose | 5 ml | 25 ml |
| Noble agar | 2 g | 10 g |
| dH2O | 93 ml | 465 ml |
Procedure
Add agar to water, autoclave, cool slightly (about 5-10 min at RT); pre-warm 50X VBG salts to 50 – 55 ºC. While gently mixing, slowly aseptically add VBG salts and glucose, mix and pour.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
