1M Tris-HCl, pH 7.5
- 121.12 g Tris base
- 800 mL dH2O
Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- If you overshoot the pH, don't add another base. You can add more Tris, but it will change the final concentration.