Gill:Tris-HCl

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(New page: 1M Tris-HCl, pH 7.5 ==Materials== 1000 mL: *Tris base 121.12 g *dH<sub>2</sub>O 800 mL *HCl ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to...)
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1000 mL:
1000 mL:
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*Tris base 121.12 g
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*121.12 g Tris base
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*dH<sub>2</sub>O 800 mL
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*800 mL dH<sub>2</sub>O
*HCl
*HCl

Revision as of 15:57, 23 April 2013

1M Tris-HCl, pH 7.5

Materials

1000 mL:

  • 121.12 g Tris base
  • 800 mL dH2O
  • HCl

Procedure

Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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