Gill:Tris-HCl: Difference between revisions
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(New page: 1M Tris-HCl, pH 7.5 ==Materials== 1000 mL: *Tris base 121.12 g *dH<sub>2</sub>O 800 mL *HCl ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to...) |
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Revision as of 12:51, 23 April 2013
1M Tris-HCl, pH 7.5
Materials
1000 mL:
- Tris base 121.12 g
- dH2O 800 mL
- HCl
Procedure
Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
