Gill:Tris-HCl: Difference between revisions
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(New page: 1M Tris-HCl, pH 7.5 ==Materials== 1000 mL: *Tris base 121.12 g *dH<sub>2</sub>O 800 mL *HCl ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to...) |
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1000 mL: | 1000 mL: | ||
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*dH<sub>2</sub>O | *800 mL dH<sub>2</sub>O | ||
*HCl | *HCl | ||
Revision as of 13:57, 23 April 2013
1M Tris-HCl, pH 7.5
Materials
1000 mL:
- 121.12 g Tris base
- 800 mL dH2O
- HCl
Procedure
Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
