Gill:TSB (Tryptone Soy Broth)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Materials== 500 mL: *TSB (Bacto) 15 g *dH<sub>2</sub>O 500 ml 1000 mL: *TSB (Bacto) 30 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml i...)
Current revision (11:29, 1 May 2013) (view source)
 
(One intermediate revision not shown.)
Line 3: Line 3:
500 mL:
500 mL:
-
*TSB (Bacto) 15 g
+
*15 g TSB (Bacto)
-
*dH<sub>2</sub>O 500 ml
+
*500 ml dH<sub>2</sub>O
1000 mL:
1000 mL:
-
*TSB (Bacto) 30 g
+
*30 g TSB (Bacto)
-
*dH<sub>2</sub>O 1000 ml
+
*1000 ml dH<sub>2</sub>O
==Procedure==
==Procedure==
Line 16: Line 16:
==Notes==
==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 +
 +
* Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.
[[Category:Protocol]]
[[Category:Protocol]]

Current revision

Materials

500 mL:

  • 15 g TSB (Bacto)
  • 500 ml dH2O

1000 mL:

  • 30 g TSB (Bacto)
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.
Personal tools