Gill:TSB (Tryptone Soy Broth)

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(New page: ==Materials== 500 mL: *TSB (Bacto) 15 g *dH<sub>2</sub>O 500 ml 1000 mL: *TSB (Bacto) 30 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml i...)
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Line 3: Line 3:
500 mL:
500 mL:
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*TSB (Bacto) 15 g
+
*15 g TSB (Bacto)
-
*dH<sub>2</sub>O 500 ml
+
*500 ml dH<sub>2</sub>O
1000 mL:
1000 mL:
-
*TSB (Bacto) 30 g
+
*30 g TSB (Bacto)
-
*dH<sub>2</sub>O 1000 ml
+
*1000 ml dH<sub>2</sub>O
==Procedure==
==Procedure==

Revision as of 16:04, 23 April 2013

Materials

500 mL:

  • 15 g TSB (Bacto)
  • 500 ml dH2O

1000 mL:

  • 30 g TSB (Bacto)
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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