Gill:TSB (Tryptone Soy Broth): Difference between revisions
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==Notes== | ==Notes== | ||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
* Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass. | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
Latest revision as of 08:29, 1 May 2013
Materials
500 mL:
- 15 g TSB (Bacto)
- 500 ml dH2O
1000 mL:
- 30 g TSB (Bacto)
- 1000 ml dH2O
Procedure
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.
