Gill:TSB (Tryptone Soy Broth): Difference between revisions
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(New page: ==Materials== 500 mL: *TSB (Bacto) 15 g *dH<sub>2</sub>O 500 ml 1000 mL: *TSB (Bacto) 30 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml i...) |
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500 mL: | 500 mL: | ||
*TSB (Bacto) | *15 g TSB (Bacto) | ||
*dH<sub>2</sub>O | *500 ml dH<sub>2</sub>O | ||
1000 mL: | 1000 mL: | ||
*TSB (Bacto) | *30 g TSB (Bacto) | ||
*dH<sub>2</sub>O | *1000 ml dH<sub>2</sub>O | ||
==Procedure== | ==Procedure== | ||
| Line 16: | Line 16: | ||
==Notes== | ==Notes== | ||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
* Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass. | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
Latest revision as of 08:29, 1 May 2013
Materials
500 mL:
- 15 g TSB (Bacto)
- 500 ml dH2O
1000 mL:
- 30 g TSB (Bacto)
- 1000 ml dH2O
Procedure
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.
